Circ_cgga162在套细胞淋巴瘤患者中的表达及意义

目的:探讨Circ_cgga162在套细胞淋巴瘤(mantle cell lymphoma,MCL)患者血清中的表达,并分析其作为预后生物标志物的潜力。方法:采用实时定量聚合酶链反应(QRT-PCR)检测86例套细胞淋巴瘤患者和50例淋巴结反应性增生(RH)患者血清中Circ_cgga162的表达,分析Circ_cgga162表达与临床病理特征的关系。使用SPSS软件,采用单因素-生存分析法(Kaplan-Meier)分析Circ_cgga162的表达与患者无进展生存时间及总生存时间之间的关系;单因素及多因素分析Circ_cgga162表达与患者临床预后的关系。结果:MCL患者血清中Circ_cgga162的表达水平明显高于RH组(P<0.01)。单因素分析提示,Circ_cgga162的表达与患者的年龄、性别、B症状、LDH均无明显关系(P>0.05);而与淋巴瘤国际预后指数(MCL International Prognostic Index,IPI)、Ann Arbor分期、骨髓浸润及Ki67的表达均明显相关(P<0.05)。Kaplan-Meier分析显示,Circ_cgga162高表达患者的无进展生存时间及总生存期明显短于低表达的患者(P<0.001)。单因素分析提示,Ann Arbor分期、Circ_cgga162表达、MIPI、骨髓浸润及Ki67均是影响MCL患者预后的因素(P<0.05);多因素Cox回归分析发现,Ann Arbor分期、Circ_cgga162表达及MIPI均是影响MCL患者预后的独立因素(P<0.05)。结论:Circ_cgga162在MCL患者组织中高表达,与患者的预后相关,可作为MCL患者潜在的预后标志物和治疗靶点。

Relationship of Expression of Circ_cgga162 with the Prognosis of Patients with Mantle Cell Lymphoma

Objective:To investigate the expression of Circ_cgga162 in serum of mantle cell lymphoma(MCL)patients and analyze its potential as a prognostic biomarker.Methods:The expression of Circ_cgga162 in 86 cases of mantle cell lymphoma and 50 cases of lymph node reactive hyperplasia(RH)were detected by real-time quantitative polymerase chain reaction(qRT-PCR).The relationship between the expression of Circ_cgga162 and clinicopathological features was analyzed by univariate analysis.The relationship of Circ_cgga162 expression with progression-free survival time and overall survival time was analyzed by Kaplan-Meier.The relationship between expression of Circ_cgga162 and prognosis of patients was analyzed by univariate and multivariate analysis.Results:The expression level of Circ_cgga162 in MCL patients was significantly higher than that in control(RH)group(P<0.01).The expression of Circ_cgga162 not correlated with age,gender,B symptoms and LDH(all P>0.05),but correlated with the expression of MCL International Prognostic Index(IPI),Ann Arbor stage,bone marrow infiltration and Ki67(all P<0.05).In addition,Kaplan-Meier analysis showed that the progression-free survival time and overall survival time of the MCL patients with high expression of Circ_cgga162 were significantly shorter than those of the MCL patients with low expression(P<0.01).Univariate analysis showed that Ann Arbor stage,Circ_cgga162 expression,MIPI,bone marrow infiltration and Ki67 were the prognostic factors for MCL patients(all P<0.05).Multivariate Cox regression analysis showed that Ann Arbor stage,Circ_cgga162 expression and MIPI were independent factors affecting the prognosis of MCL patients(all P<0.05).Conclusion:Circ_cgga162 is highly expressed in serum of patients MCL,which relates with the prognosis of MCL patients.Circ_cgga162 can be used as a potential prognostic marker and therapeutic target for MCL patients.