毛菊苣提取物对小鼠肝纤维化的保护作用

背景肝纤维化是肝硬化发生的基础病理过程,逆转和治疗肝纤维化是防止肝硬化发生的重要治疗手段.毛菊苣,作为传统中药材,已知其保肝作用,本文通过动物实验验证并进一步探索其发挥作用的有效部位.目的研究毛菊苣提取物:CG-Ⅰ(95%乙醇提取物)、CG-Ⅱ(70%乙醇提取物)、CG-Ⅲ(50%乙醇提取物)、CG-Ⅳ(水提取物)对小鼠肝纤维化的保护作用.方法采用10%食用酒精代饮用水,背部皮下注射20%CCl4造肝纤维化模型,造模成功后分为空白对照组、模型组、药物治疗组(CG-Ⅰ组、CG-Ⅱ组、CG-Ⅲ组、CG-Ⅳ组),考察肝脏系数、脾脏系数,血清谷草转氨酶(aspartate aminotransferase,AST)、谷丙转氨酶(alanine aminotransferase,ALT)、肝组织碱性磷酸酶(alkaline phosphatase,AKP)、乳酸脱氢酶(lactate dehydrogenase,LDH)、谷光甘肽过氧化物酶(glutathione peroxidase,GSH-Px)的水平,并观察肝脏病理组织学变化.结果与空白对照组相比,模型组小鼠血清及肝组织中的AST、ALT、AKP及LDH水平都显著升高,说明造模成功;与模型组相比,CG-Ⅲ组可以降低脾脏系数(P<0.05);药物治疗组均可降低小鼠血清中AST、ALT的水平(P<0.01);药物治疗组除了CG-Ⅲ组均可以升高小鼠肝组织中GSH-Px的活性水平(P<0.01或P<0.05);药物治疗组CG-Ⅰ和CG-Ⅱ可降低小鼠肝组织中AKP、LDH的含量,具有极显著性差异(P<0.01);通过HE染色和Masson染色切片,可以看出药物治疗组均可不同程度地改善肝脏病理组织纤维化,而CG-Ⅰ组和CG-Ⅱ组效果显著.结论维药毛菊苣提取物对小鼠肝纤维化具有一定的保护作用,其中CG-Ⅰ组和CG-Ⅱ组即95%和70%乙醇提取物组的治疗效果最佳.

Protective effects of Cichorium glandulosum Boiss extracts against liver fibrosis in mice

BACKGROUND Hepatic fibrosis is the basic pathological process of cirrhosis.Chicory,as a traditional Chinese medicine,has been known to be able to protect the liver.In this study,animal experiments were conducted to verify and further explore the hepatoprotective effects of chicory.AIM To investigate the hepatoprotective effects of Cichorium glandulosum Boiss extracts CG-Ⅰ(95%ethanol extract),CG-Ⅱ(70%ethanol extract),CG-Ⅲ(50%ethanol extract),and CG-Ⅳ(water extract)in mice with alcohol-induced liver fibrosis.METHODS Liver fibrosis was induced in mice by subcutaneous injection of 20%carbon tetrachloride and administration of 10%edible alcohol in drinking water.The mice were divided into a blank control group,a model group,and four drug groups(CG-Ⅰ,CG-Ⅱ,CG-Ⅲ,and CG-Ⅳgroups).The liver and spleen indexes;aspartate aminotransferase(AST)and alanine aminotransferase(ALT)in serum;alkaline phosphatase(AKP),lactate dehydrogenase(LDH),and glutathione peroxidase(GSH-Px)in liver homogenate;and the histopathological changes of the liver were assayed.RESULTS Compared with the blank control group,AST,ALT,AKP and LDH levels in serum and liver tissues of the model group were significantly increased,indicating that the model was successfully established.Compared with the model group,the spleen coefficient was significantly decreased in the CG-Ⅲgroup(P<0.05);serum ALT and AST were significantly decreased in all the drug groups(P<0.01);the activities of GSH-Px were significantly increased in all the drug groups except the CG-Ⅲgroup(P<0.01 or P<0.05);serum AKP and LDH were significantly decreased in the CG-Ⅰand CG-Ⅱgroups(P<0.01);and liver pathology was improved in all the drug groups,with the improvement more remarkable in the CG-Ⅰand CG-Ⅱgroups.CONCLUSION CG-Ⅰand CG-Ⅱhave significant protective effects against alcohol-induced liver fibrosis in mice.