香砂六君子汤对慢性萎缩性胃炎大鼠胃组织PI3K信号通路相关因子表达的影响

目的观察香砂六君子汤对慢性萎缩性胃炎(CAG)大鼠胃组织血管内皮生长因子(VEGF)、磷酸酶张力蛋白同源物基因(PTEN)、磷脂酰肌醇3-激酶(PI3K)、蛋白激酶B(AKT)的影响,探讨该复方防治CAG的作用机制。方法将120只Wistar大鼠分为空白组和模型组,采用综合法制作CAG动物模型,将成模大鼠分为模型组、维酶素组和香砂六君子汤高、中、低剂量组,空白组和模型组大鼠给予10 mL/(kg·d)蒸馏水灌胃,香砂六君子汤高、中、低剂量组分别给予24、12、6 g/(kg·d)香砂六君子汤灌胃,维酶素组给予0.30 g/(kg·d)维酶素灌胃,连续120 d。ELISA检测大鼠胃组织VEGF含量,RT-PCR和Western blot分别检测大鼠胃组织PTEN、PI3K、AKT基因和蛋白的表达。结果与空白组比较,模型组大鼠一般状况较差,胃黏膜变薄,上皮细胞及腺体排列紊乱、稀疏,腺体数目明显减少,黏膜肌层增厚向固有层延伸,可见大量炎性细胞浸润,胃组织PTEN基因及蛋白表达显著降低(P<0.01),VEGF含量和AKT、PI3K基因及蛋白表达显著升高(P<0.01);与模型组比较,香砂六君子汤高剂量组大鼠胃组织PTEN基因表达显著升高(P<0.01),VEGF含量和AKT、PI3K基因及蛋白表达显著降低(P<0.01)。结论香砂六君子汤通过改善模型大鼠生存状况及胃黏膜病理状态,上调胃组织PTEN表达,下调VEGF、AKT、PI3K表达,发挥治疗CAG的作用。

Effects of Xiangsha Liujunzi Decoction on PI3K Signaling Pathway Correlation Factor Expression in Gastric Tissue of Rats with Chronic Atrophic Gastritis

Objective To observe the effects of Xiangsha Liujunzi Decoction on vascular endothelial growth factor(VEGF),phosphatase tensin protein homologue(PTEN),phosphatidylinositol 3-kinase(PI3K)and protein kinase B(AKT)in gastric tissue of rats with chronic atrophic gastritis(CAG);To discuss the mechanism of this compound for prevention and treatment of CAG.Methods Totally 120 SPF Wistar rats were randomly divided into blank group and model group.CAG animal models were reproduced by comprehensive method.The model rats were randomly divided into model group,vitacoenzyme tablets group and Xiangsha Liujunzi Decoction high-,medium-and low-dosage groups.The blank group and model group were given 10 mL/(kg·d)distilled water for gavage,and Xiangsha Liujunzi Decoction high-,medium-and low-dosage groups were given 24 g/(kg d),12 g/(kg d),and 6 g/(kg·d)Xiangsha Liujunzi Decoction,respectively,for gavage.Vitacoenzyme tablets group was given 0.30 g/(kg·d)vitacoenzyme tablets,for consecutive 120 d.The content of VEGF in gastric tissue was detected by ELISA,and the gene and protein expressions of PI3K,PTEN and AKT were detected by RT-PCR and Western blot,respectively.Results Compared with the blank group,the general survival condition of the model group was worse;the gastric mucosa of the model group was thinner,the arrangement of epithelial cells and glands was disordered and sparse,the number of glands was significantly reduced,the muscular layer of the mucosa was thicker and extended to the lamina propria,a large number of inflammatory infiltration could be seen,PTEN gene and protein expression in gastric tissue were significantly reduced(P<0.01),and the content of VEGF and the gene and protein expressions of AKT and PI3K in gastric tissues significantly increased(P<0.01).Compared with model group,the gene expression PTEN in gastric tissue of rats in Xiangsha Liujunzi Decoction high-dosage group was significantly higher(P<0.01),and the content of VEGF and gene and protein expressions of AKT and PI3K in gastric tissues significantly decreased(P<0.01).Conclusion Xiangsha Liujunzi Decoction can exert the therapeutic effect on CAG by improving the general survival of the model rats and the pathological state of gastric mucosa,up-regulating the expression of PTEN in gastric tissue,down-regulating the expression of VEGF,AKT and PI3K.