原发性肝细胞癌血清中miR-888-5p的表达及临床意义

背景miR-888-5p高表达于原发性肝细胞癌(hepatocellular carcinoma,HCC)组织及HCC细胞系,可促进肿瘤细胞侵袭及转移,与疾病分期及预后不良相关.然而HCC患者血清中miR-888-5p的表达水平尚未被检测,血清miR-888-5p对于HCC诊断及预后判断相关价值尚未被评估.目的检测HCC患者血清中miR-888-5p表达水平,探究其对HCC的诊断价值及与临床特征之间的关系.方法收集68例HCC患者、46例慢性乙型病毒性肝炎(chronic hepatitis B,CHB)患者、43例肝硬化(liver cirrhosis,LC)患者及40例同期健康体检者,实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,qRT-PCR)法检测血清中miR-888-5p表达量,受试者工作特征(receiver operating characteristic,ROC)曲线评估其诊断HCC的价值,并分析与HCC临床特征之间的关系.结果HCC患者血清中miR-888-5p表达量较CHB、LC及健康者显著上调(P<0.05).ROC曲线提示,联合检测ROC曲线下面积(area under curve,AUC)为0.907,敏感性91.18%,特异性72.50%)对HCC诊断价值优于分别检测血清miR-888-5p(AUC=0.737,敏感性79.41%,特异性62.50%)及甲胎蛋白(alpha-fetoprotein,AFP)(AUC=0.819,敏感性73.53%,特异性97.50%),且对AFP阴性HCC也具有较高的诊断价值:AUC=0.793,敏感性90.90%,特异性62.50%.此外,miR-888-5p表达水平与HCC肺转移相关(P=0.01).结论血清miR-888-5p对HCC及AFP阴性HCC具有较高诊断价值,与AFP联合检测诊断价值更高.miR-888-5p表达水平与HCC肺转移相关,有望成为HCC早期诊断及评估预后的新型血清学分子标志物.

Clinical significance of serum miR-888-5p in patients with primary hepatocellular carcinoma

BACKGROUND MiR-888-5p is highly expressed in hepatocellular carcinoma(HCC)tissues and cell lines,and it can promote tumor invasion and metastasis and is related to disease stage and poor prognosis.However,the clinical value of serum miR-888-5p levels in the diagnosis and prognosis of HCC remains unclear.AIM To detect serum miR-888-5p in patients with HCC and investigate its diagnostic value and correlation with clinical characteristics of HCC.METHODS A total of 197 subjects were enrolled,consisting of 68 HCC patients,46 chronic hepatitis B(CHB)patients,43 liver cirrhosis(LC)patients,and 40 healthy volunteers.Serum miR-888-5p levels were measured by quantitative real-time polymerase chain reaction(qRT-PCR).The diagnostic value of miR-888-5p and its correlation with clinical features were assessed.RESULTS Compared with CHB patients,LC patients,and healthy controls,serum miR-888-5p increased significantly in HCC patients(P<0.05).Receiver operating characteristic curve(ROC)analysis demonstrated that the area under the ROC curve(AUC)of miR-888-5p combined with alpha-fetoprotein(AFP)in discriminating HCC patients from healthy controls was 0.907(sensitivity:91.18%;specificity:72.50%),higher than that of either AFP(AUC=0.819;sensitivity:73.53%;specificity:97.50%)or miR-888-5p alone(AUC=0.737;sensitivity:79.41%;specificity:62.50%).Serum miR-888-5p maintained its diagnostic efficiency in AFP negative HCC patients with an AUC of 0.793(sensitivity:90.90%;specificity:62.50%).Serum miR-888-5p levels were significantly associated with pulmonary metastasis(P=0.01)of HCC.CONCLUSION Serum miR-888-5p has high diagnostic value for HCC,and combined detection of serum miR-888-5p and AFP could improve the diagnostic efficiency.MiR-888-5p also has good diagnostic value for AFP negative HCC,and it is closely related to lung metastasis of HCC.MiR-888-5p is expected to become a new serum marker for early diagnosis and prognosis evaluation of HCC.