异基因造血干细胞移植后供受者嵌合状态自动分析和监测平台的开发和应用

目的:开发一套基于短串联重复序列(STR)标记和毛细管电泳法进行异基因造血干细胞移植(allo-HSCT)后嵌合率检测时可自动数据分析和报告的工具软件,以提高工作效率。方法:采用Apache+MySQL+PHP+HTML5技术搭建数据库和访问界面,根据基于STR和毛细管电泳法判别STR位点基因型和计算嵌合率的规则设定分析逻辑和流程。抽取100例allo-HSCT患者的STR分型和移植后194次嵌合率检测数据,应用此平台进行自动STR位点分型鉴定、嵌合率计算及生成报告。结果:所搭建的分析平台可实现自定义STR位点、基因型智能识别、嵌合比例自动分析、检测信息数据库化管理,可自动生成含该患者多次检测、多种不同成分细胞嵌合比例结果的整合报告和嵌合比例趋势图等功能,并且可通过浏览器多用户同时访问和使用。应用该平台自动分析和有经验人员的手工分析结果完全一致,并且通过自动化减少了手工分析失误的可能。使用该平台自动分析所用时间约为手工分析的1/6-1/5。结论:本研究搭建的嵌合率自动分析平台运行稳定、位点分型和嵌合率分析结果可信,可提高工作效率和报告内涵,具有推广应用价值。

Development and Application of Automatic Analysis and Surveillance Platform for Chrimerism in Donors and Recipients after Allo-HSCT

Objective:To develop an automated chimeric analysis and reporting platform based on short tandem repeat(STR)and capillary electrophoresis methods for allogeneic hematopoietic stem cell transplantation(allo-HSCT)so as to improve work efficiency.Methods:Apache,MySQL,PHP and HTML5 were used to build the database and interface.The STR locus geno typing and chimeric analysis logic and flow were set up on the basis of STR rules and capillary electrophoresis.STR genotyping and 194 times of chimeric testing data of 100 patients after allo-HSCT were used to test the platform for automatic STR locus genotyping,chimeric calculation and report generation.Results:The established platform could realize the functions of STR locus customization,STR genotype determination,automatic chimeric analysis,and detection information database management,which can automatically generate an integrated report including multiple sequential chimeric results and trend graphs for the same patient and can be accessed and used simultaneously by different users through different browser interfaces.The results of automated analysis by the platform are completely consistent with that of manual analysis by experienced technicians,and the possibility of manual analysis error is reduced through automation.The time required for automatic analysis using this platform is approximately 1/6-1/5 of manual analysis.Conclusion:The automatic analysis platform built in this study is operation stable and reliable in analysis results,which can improve work efficiency and report connotation,thus worthing popularized and applicable.