高效液相色谱法测定别嘌醇有关物质

目的：建立别嘌醇高效液相色谱法有关物质测定。方法：采用HPLC—Uv法，并考察色谱条件，优化分离效果，以十八烷基硅烷键合硅胶为填充剂；以甲醇-1．25班磷酸二氢钾溶液（20：80）为流动相；检测波长为230nm。结果：杂质能与别嘌醇得到有效分离，分离度大于1．5；检测限为0．6ng，精密度良好（RSD为1．89％）。结论：高效液相色谱法可用于测定别嘌醇中的有关物质。

Determination of allopurinol related substances by RP-HPLC with ultraviolet detection

Objective：To establish a reversed-phase high performance iiquid chromatography （RP-HPLC） for determination of allopurinol related substances. Methods ：The specific and stable RP-HPLC with ultraviolet detection was employed,and the chromatographic conditions were investigated to optimize the separation. The chromatography was accomplished on C18, and the moble phase con- sisted of methanol-1.25 g/L potassium dihydrogen phosphoric acid,and the detection wavelength was set at 230 nm. Results：All the related substances gained a completely chromatographic separation with the principal peak.The limit of detection（LOD） was 0.6 ng based on S/N greater than 3：1.The sensitivity was much good（RSD〈1.89%. Conclusion：The method is adopted for determination of allopurinol related substances.