姜黄素聚合物胶束的制备及体外评价

目的:制备姜黄素的Soluplus聚合物胶束,并对其进行体外评价。方法:采用薄膜分散法制备姜黄素聚合物胶束;采用粒径测定仪、透射电镜、X-射线衍射(XRD)对其进行表征;采用紫外分光光度法测定胶束的包封率和载药量;采用动态膜透析法考察载药胶束的体外释药特性。结果:薄膜分散法制备的胶束呈球形或类球形,平均粒径为(65.54±2.57)nm,平均包封率为(87.73±2.94)%,平均载药量(7.96±2.13)%;XRD结果表明姜黄素以无定型状态或分子状态包载在聚合物胶束中;体外释放结果表明姜黄素的soluplus聚合物胶束具有缓释作用。结论:该胶束制备工艺简单,其粒径、包封率、载药量可控,具有缓释作用。

Preparation of curcumin-loaded polymeric micelles and in vitro evaluation

OBJECTIVE To prepare curcumin-loaded soluplus polymeric micelles (Cur-PMs) and perform evaluation in vitro.METHODS Cur-PMs were prepared by film dispersion method using an amphiphilic copolymer soluplus.The Cur-PMs were characterized by dynamic light scattering,transmission electron microscopy (TEM),X-ray diffraction (XRD).Entrapment efficiency (EE) and drug loading (DL) were determinated by UV.The release curve was determined by dialysis.RESULTS The Cur-PMs observed by TEM were spherical with a mean particle size of (65.54±2.57)nm,the encapsulation efficiency and drug loading of Cur-PMs were (87.73±2.94)% and (7.96±2.13)%,respectively.X-ray diffraction showed that curcumin was in an amorphous or molecular form in the PMs.Release in vitro showed that Cur-PMs could slow down the release rate.CONCLUSION The preparation process is simple,with controllable particle size,entrapment efficiency and drug loading,and can delay drug release.