胃癌前哨淋巴结活检技术及其微转移检测应用研究

目的 探讨前哨淋巴结活检技术在胃癌中应用及流式细胞术对胃癌前哨淋巴结微转移检测的可行性。方法 南通大学附属医院普外科2006年6月至2007年6月对60例胃癌病人术中应用亚甲蓝定位活检前哨淋巴结（SLN），术后行HE染色及流式细胞术检测。结果 60例中56例找到SLN，检出率为93.3%，SLN组织学状态能比较准确预测胃癌区域淋巴结转移状况, 在T1、T2期中更为明显。HE发现49枚/24例SLN转移，流式细胞术发现71枚/37例SLN转移，即22枚/13例SLN存在微转移。结论 亚甲蓝行前哨淋巴结活检（SLNB）在胃癌中是可行的，特别在T1、T2期中更具有重要意义，流式细胞术结合SLNB检测胃癌的淋巴结微转移具有较高的检出率, 具有临床可行性。

Clinical application of Sentinel lymph node biopsy technology in gastric cancer and it's micrometastasis detection

Clinical application of Sentinel lymph node biopsy technology in gastric cancer and it′s micrometastasis detection YOU Jian-Quan*，MA Li-lin .*Department of General Surgery, Taizhou People’s Hospital,Taizhou 225300, China Abstract Objective To investigate the micrometastasis of sentinel lymph node and the feasibility of sentinel lymph node biopsy in gastric cancer. Methods Sixty patients with gastric cancer intraoperatvely underwent setinel lymph node biopsy with methylene. All SLNs were studied with HE and FCM (Flow cytometry) technique,respectively. ResuLts The stained SLNs couLd be identified in 56(93.3%) of 60 patients. 49 SLNs were found to have lymph node metastases in 23 patients by HE, however, 71 SLNs were found to have lymph node metastases in 27 patients by FCM.That is to say: 22 SLNs micrometastases were detected in 13 patients. In the patients with gastric cancer of T1 and T2 groups, SLN can much more accurately predict the LNs status. Conclusion Intraoperatvely SLNB with methylene in gastric cancer is more feasible in T1 and T2 groups; FCM binding SLNB can detect lymph node micrometastasis in gastric cancer as many as possible that is feasible and prospective.