| 细胞因子及其组合对脐血基质细胞集落形成作用的比较 |
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| 引用本文： | 张曦,陈幸华,刘林,彭贤贵,孔佩艳,刘红,张怡,高蕾,钟永明,王庆余.细胞因子及其组合对脐血基质细胞集落形成作用的比较[J].重庆医学,2003,32(5):550-552. |
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| 作者姓名： | 张曦 陈幸华 刘林 彭贤贵 孔佩艳 刘红 张怡 高蕾 钟永明 王庆余 |
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| 作者单位： | 第三军医大学新桥医院血液科,重庆,400037 |
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| 基金项目： | 国家自然科学基金资助项目 (30 0 70 32 7) |
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| 摘 要： | 目的 观察细胞因子SCF、IL—3、BFGF及其不同组合对脐带血基质细胞集落形成的作用。方法 应用体外脐带血基质细胞贴壁培养的方法，分别加入不同的细胞因子，进行培养3周时的基质细胞集落计数和培养4周时贴壁细胞的增殖状态观察。结果 SCF BFGF、SCF BFGF IL—3对脐带血基质细胞的增殖具有明显的促进作用。结论 体外加入某些细胞生长因子对脐带血基质细胞具有明显的扩增作用，提示运用脐带血作为新的基质细胞来源在体外细胞因子组合的作用下扩增后进行回输，有望成为促进造血损伤修复的新措施。
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| 关 键 词： | 基质细胞 细胞因子 扩增 脐带血 |
| 文章编号： | 1671-8348(2003)05-0550-03 |
The effects of some cytokines on expansion of cord stromal cells |
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| ZHANG Xi,CHENG Xing hua,LIU lin,et al..The effects of some cytokines on expansion of cord stromal cells[J].Chongqing Medical Journal,2003,32(5):550-552. |
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| Authors: | ZHANG Xi CHENG Xing hua LIU lin |
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| Abstract: | Objective To study the effects of recombinant cytokines SCF?IL 3?BFGF on cord stromal cell colony.Methods Using the methods of ex vivo culture of cord stromal cells,and add the different cytokine(s)individually.At the third and the foruth weekend,the number of cord stromal cell colony was counted and the morphology of the stromal cells in culture were observed,respectively.Results The significant promotin effects on the growth of cord stromal cells were observed when SCF+BFGF?SCF+BFGF+IL 3 were added.Conclusion the results indicate that the some hematopoietic growth factors also have promoting effects on the ex vivo expansion of cord stromal cells,and which maybe have an important significance on the repairing of hematopoietic injury. |
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| Keywords: | stromal cells Cytokines ex vivo expansionl Cord |
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