正交设计法优选白芷的提取工艺

目的以欧前胡素、异欧前胡素的提取总量为考察指标,对影响香豆素类化合物提取工艺的因素进行研究,优选白芷的最佳提取工艺。方法采用RP HPLC法,AccusilC18柱( 2 5 0mm×4 6mm ,10 μm) ,流动相为甲醇 水(体积比6 5∶35 ) ;检测波长2 5 1nm。结果欧前胡素与异欧前胡素分别在0 76 8～19 2 0mg·L-1(r =0 9997)、0 2～16 0mg·L-1(r =0 9998)内与峰面积呈良好的线性关系(n =6 ) ,方法回收率(n =9)分别为96 8%、98 0 % ,RSD分别为2 0 %、1 9%。结论白芷的最佳提取工艺为A3 B2 C3 ,即用8倍量的95 % (w)的乙醇提取3次,每次4h。

Studies on the process for extraction of Radix Angelicae Dahuricae by orthogonal experiments

Objective To optimize the conditions for the extraction of Angelica dahurica Methods A Accusil C 18 column(250 mm×4.6 mm,10 μm)with a solvent system consisting of methanol-water(V∶V=65∶35)was used,the detecting wavelength was set at 251 nm and the column temperature was 35℃.Results The linear ranges of imperatorin and isoimperatorin were 0.768～ 19.20 mg·L~ -1 (correlation coefficient r= 0.999 7 )and 0.2～ 16.0 mg·L~ -1 (correlation coefficient r= 0.999 8 ),respectively.The average recoveries of imperatorin and isoimperatorin were 96.8%,98.0%,with RSD being of 2.0%,1.9%(n=9),respectively.Conclusions The optimum conditions for the extraction of Angelica dahurica is A 3B 2C 3.That is,adding eight times of 95% alcohol,refluxing and extracting for 4 h ,totally extracting 3 times.