Time-resolved fluoroimmunoassay of pancreatic phospholipase A2 |
| |
| Authors: | T. J. Nevalainen J. U. Eskola |
| |
| Affiliation: | (1) Department of Pathology, University of Turku, Finland;(2) Department of Clinical Chemistry, University of Turku, Finland |
| |
| Abstract: | Summary We have developed an immunoassay for human pancreatic secretory phospholipase A2 based on time-resolved fluorescence. The labeled antibody technique in combination with the time-resolved fluorometric detection of the Europium label, which essentially eliminates all background fluorescence, resulted in a high sensitivity and a wide linear range. Our assay is a one-incubation, multi-site, solid-phase assay on polystyrene micro-titer strips, even though a polyclonal antibody was used. Increased levels of immunoreactive phospholipase A2 are found by this assay in sera of patients suffering from acute pancreatitis. |
| |
| Keywords: | Acute pancreatitis Europium Immunoassays Pancreatic disease Solid-phase assay |
| 本文献已被 SpringerLink 等数据库收录！ |
|
