胰岛素样生长因子1的体内表达对BXSB小鼠发病影响的研究

目的 初步研究人胰岛素样生长因子1(hIGF-1)对系统性红斑狼疮ＢＸＳＢ小鼠发病的影响。方法 用肌肉介导的电脉冲转基因技术，使得含有hIGF-1cDNA的重组真核表达质粒在小鼠体内表达，然后系统检测自身免疫相关性实验指标。结果 虽然hIGF-1促进雄性ＢＸＳＢ小鼠ＩgG类型抗ＤＮＡ抗体的产生，但是明显阻止其尿蛋白浓度的继续升高，抑制巨噬细胞分泌IL-1以及降低脾脏重量。结论 IGF-1具有减缓雄性BXSB小鼠狼疮性肾炎的作用。其作用机制之一可能与巨噬细胞分泌炎性介质的功能下降有关。

Experimental study on the effect of human insulin like growth factor 1 on the development of systemic lupus erythematosus in BXSB mouse in vivo

Objective To study the in vivo effects of human insulin like growth factor 1 (hIGF 1) on the development of systemic lupus erythematosus of BXSB mouse. Methods Recombinant eukaryotic expression plasmid for hIGF 1 (pcDNA3 IGF 1) was transferred and expressed in lupus prone BXSB mice with the technique of muscle mediated transgene of electric pulse. Autoimmune related assays were carried out and analyzed systemically. Results hIGF 1 increased the production of IgG anti DNA antibody in male BXSB mouse, but strikingly retarded the continueous elevation of proteinuric concentration as aging, followed by the suppression of macrophage secretion for IL 1 and the weight decrease of spleen. Conclusion IGF 1 may have the ability of alleviating severity of lupus glomerulonephritis of male BXSB mouse, which is possibly associated with the inhibition of inflammation mediators secreted by macrophage.