筛选妊娠糖尿病巨大儿大鼠模型的新方法

目的建立妊娠糖尿病巨大儿大鼠模型。方法SD雌性大鼠腹腔注射链脲佐菌素（streptozotoein，STZ）25mg／kg，之后与同系雄性大鼠合笼见栓者定为孕0d。孕6d测定血糖高出孕前20％者为模型组（M组），与正常对照组（C组）继续妊娠至孕21d行剖腹产术，分离胎鼠。结果M组胎鼠体重、身长、尾长均显著大于C组（均P〈0．001）、胎鼠血糖高于C组（P〈0．001）。结论腹腔注射25mg／kg STZ后挑选孕6d血糖高于孕前20％者可复制妊娠糖尿病巨大儿大鼠模型。

Screening of Gestational Diabetes Mellitus Models with Macrosomia in Rats: A New Approach

Objective To establish gestational diabetes mellitus model with macrosomia in rats Methods 7 days after being injected STZ 25mg/kg intraperitoneally,Female SD rats were mated with the male.On the day 6 of conception,plasma glucose(PG) levels were determined and those whose PG level increased by 20% than that before pregnancy were selected as the Model(M) group.All rats were c-sectioned on the day 21 of pregnancy to observe the fetuses.Results Body weights,body lengths,tail lengths of the offspring in M group were significantly heavier or longer than those in C group(P<0.001).Fetal plasma glucose concentrations in M group were higher than those in C group and the statistic difference was noted(P<0.001).Conclusion 7 days after being injected STZ 25 mg/kg intraperitoneally,GDM rats with 20% increase of plasma glucose levels on the day 6 of conception than before pregnancy can produce macrosomia.