Fascin1基因表达对胃癌细胞系MKN45增殖与凋亡的影响

目的：探讨fascin1基因表达对胃癌细胞系MKN45增殖与凋亡的影响及其意义。方法：利用RNA interference(RNAi)技术稳定沉默fascin1基因的表达，Western blotting及RT-PCR检测干扰效率，用MTT比色法和平板克隆形成实验检测转染前后细胞体外增殖能力，用流式细胞术（FCM）及Hochest33258荧光染色法检测细胞凋亡的变化。结果：在稳定转染fascin1干扰质粒后，胃癌细胞系MKN45的fascin1表达明显下降；与未转染组和空白质粒转染组相比，fascin1干扰质粒转染组的增殖能力明显降低，但细胞凋亡变化不明显。结论：fascin1的表达与胃癌细胞系MKN45的增殖有关，而与凋亡无明显相关。

The effect of fascin 1 on proliferation and apoptosis in gastric cancer cell line MKN45

Objective:To investigate the effect of fascin 1 on proliferation and apoptosis in gastric cancer cell line MKN45. Methods :RNA interference (RNAi) was used to inhibit fascin 1 expression in human gastric cancer cell line MKN45. Expression of fascin 1 was examined by western blotting and RT-PCR. Cell growth ability was evaluated by MTT and clone formation assays. Cell apoptosis was examined by flow cytometry and Hochest 33258 staining method. Results:Knockdown of fascin1 expression in MKN45 cells resulted in decreased cellular proliferative ability, but not apoptosis. The cloning efficiency of cells transfected with small interfering RNA(siRNA) was significantly lower ［(36.5±8.1)%］ as compared to that in MKN45(79±6.3)% (P<0.05). Conclusions:Expression of fascin 1 is related to the proliferation of gastric cancer cell line MKN45, but has no significant relationship to apoptosis.