定志小丸对雌性抑郁模型大鼠海马结构的影响

目的围绕海马结构的形态学研究定志小丸的抗抑郁机制。方法40只雌性大鼠分为对照组、抑郁症模型组（模型组）、蒸馏水组（DW组）和定志小丸组（中药组），Open field法检测行为学得分，ELISA法检测血清皮质醇和雌二醇（E2）含量，HE染色和电镜技术观察海马CA3区神经元形态，免疫组化技术检测齿状回神经干细胞（NSC）增殖。结果与对照组比较，模型组和DW组行为学得分及血清E2含量显著下降，皮质醇水平升高和NSC增殖明显降低，海马CA3区神经元损伤明显；中药组行为学得分和血清E2含量无明显变化，皮质醇明显减少，NSC增殖显著增加，海马CA3区神经元基本正常。结论定志小丸能够保护海马结构，这可能是其抗抑郁机制之一。

Effect of Dingzhixiaowan on Hippocampal Formation in Depression Model Rats

Objective To study the anti-depression mechanism of Dingzhixiaowan concerning on histology of hippocampal formation. Methods 40 female rats were divided into control, depression model group （model group）, distilled water group （DW group） and Dingzhixiaowan group （Chinese herb group）. Behavior points were measured by open field method. Serum concentrations of cortisol and estradiol （E2） were checked with ELISA. HE staining and electron microscopy were applied to observe the histology of neurons in hippocampal formation. Immunohistochemistry method was employed to detect Nestin expression. Compared with the control, behavior points, serum E2 level and Nestin expression decreased obviously and serum cortisol level increased markedly, CA3 neurons were seriously damaged in model and DW groups. While behavior points, serum E2 level did not change, Nestin expression increased obviously and serum cortisol level decreased markedly, CA3 neurons were basically normal in Chinese herb groups. Dingzhixiaowan can protect hippocampal formation, which might be one of the anti-depression mechnisms.