兔胆囊结石形成过程中脂代谢和载脂蛋白变化研究

采用高胆固醇膳食诱发兔胆囊胆固醇结合模型，分别对高胆固醇膳食后１，２，３，４，周组及对照组血清脂质，载脂蛋白变化进行了研究。结果显示：１．高胆固醇膳食后１周开始出现胆囊结石２，３，和４周分别有４只，６只和７只兔出现胆囊结石；２．血清总胆固醇，甘三油酯，磷脂，低密度脂蛋白胆固醇及极低密度脂蛋白胆固醇逐渐明显升高，高密度脂蛋白胆固醇及其亚组份在降低趋势，但无显著性差异；

Change of serum lipid, apolipoprotein during cholesterol gallstone formation in rabbit model]

In order to study the formation of cholesterol gallstone through rabbit model which was induced by high cholesterol diet (HCD), we investigated the rabbits' serum lipoprotein cholesterols and apolipoprotein (apo) at 1 week (1w), 2 weeks (2w), 3 weeks (3w) and 4 weeks (4w) in comparison with those of a control group respectively. The results were as follows: (1) of 10 rabbits subjected to experiment, 4, 6, and 7 rabbits were found to have induced-cholesterol gallstones in the 2w, 3w and 4w groups respectively. (2) The serum concentrations of total cholesterol (TC), triglyceride (TG), phospholipid (PL), low density lipoprotein cholesterol (LDL-C) and very low density lipoprotein chloesterol (VLDL) increased significantly (1w, 2w, 3w and 4w groups vs control group, P < 0.05), especially in the 3w and 4w groups; the surum concentrations of high density lipoprotein cholesterol and its subfractions (HDL-C, HDL2-C, HDL3-C) decreased slightly (vs control group, P > 0.05). (3) The serum contents of apoB100, apoC II and apoC III increased significantly (vs control group, P < 0.05), especially in the 3w and 4w groups; the serum apoA I reduced gradually in 1w, 2w and 3w groups, and decreased greatly in 4w group (vs control group, P < 0.05). The results demonstrated that owing to the intake of high cholestrol diet with the passage of time, the increased concentrations of serum VLDL-C, apoB, apoC II and apoC III possibly caused an enhanced secretion of biliary cholesterol into bile; that the decreased serum apoA I level might reduce the secretion of anti-nucleating factor into bile. All of these factors and changes may play important roles during the formation of cholesterol gallstones.