HPLC法测定血竭及含血竭复方中药制剂中血竭素的含量

为制订四川金药的质量标准，采用反相高效液相色谱法测定血竭及含血竭的中成药中血竭素的含量。实验结果表明；以甲醇－０．０５ｍｍｏｌ／Ｌ磷酸二氢钠作流动相，用ＯＤＳ柱能使血竭素有很好的分离。同时利用甲醇为洗脱剂，对血竭中其余高保留组分进行洗脱，能缩短测定时间，提高工作效率。

Quantitative determination of dracorhodin in Daemonorops draco B1. and traditional Chinese medicines containing Daemonorops draco B1. by HPLC]

A reversed phase high performance liquid chromatographic mertod for the determination of Dracorhodin in Daemonorops draco B1. and Traditional Chinese Medicines containing Daemonorops draco B1. has been developed so as to set the quality standard for Sichuan Jinyao. The result showed that the components of Dracorhodin were separated by ODS column, with MeOH-0.05 mol/L NaH2PO4 (51:50) (pH = 3.10-3.15, adjusted with H2PO4) acting as the mobile phase. The other high retentive components were eluted by methanol; thus the analytical time was reduced. The method is simple and accurate.