一氧化氮介导大鼠低氧性肺动脉高压的实验研究

为了解一氧化氮（ＮＯ）在低氧性肺动脉高压中的作用，并探讨其可能机理，检测正常对照组及缺氧１、２、３周组大鼠血浆ＮＯ水平，并给缺氧２周组大鼠注入左旋精氨酸及Ｎ＾Ｇ－硝基－左旋精氨酸，观察其对大鼠血流动力学及肺病理改变的影响。结果：ＮＯ水平在缺氧１、２、３周时分别为５±２．６７μｍｏｌ／Ｌ、２．１±．０．４１μｍｏｌ／Ｌ和０．５±０．１６μｍｏｌ／Ｌ，明显低于正常对照组６．７３±１．８３μｍｏｌ／Ｌ（

Experimental study of hypoxic pulmonary hypertension induced by nitric oxide in rats]

This experiment in rats was designed to investigate the effect and mechanism of nitric oxide (NO) in the induction of hypoxic pulmonary hypertension. The plasma concentrations of NO in normal controls and in the 1-, 2- and 3-week hyporemic ventilation groups were measured. The hemodynamic and pathological changes were observed in rats of the 2-week group after bolus injection of L-Arginine and NG-nitro-L-arginine. The results showed that NO concentrations of the 1-2- and 3-week groups were 5 +/- 2.67 mumol/L 2.1 +/- 0.41 mumol/L and 0.5 +/- 0.16 mumol/L respectively, which were significantly lower than the control group's 6.73 +/- 1.83 mumol/L (P < 0.05). Bolus injection of L-Arginine 100 mg.kg-1.d-1 could relieve chronic hypoxic pulmonary hypertension and decrease the thickening of pulmonary arteries, but L-NNA could antagonize the effect of L-Arginine. This experiment demonstrates that chronic hypoxemia may decrease the release of NO and result in pulmonary hypertension. L-Arginine may be used to relieve pulmonary hypertension, but L-NNA may antagonize the effect of L-Arginine.