Increase in the Potential of Osteoblasts to Support Bone Resorption by Osteoclasts In Vitro in Response to Roughness of Bone Surface |
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Authors: | T Matsunaga H Inoue T Kojo K Hatano T Tsujisawa C Uchiyama Y Uchida |
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Institution: | (1) Second Department of Prosthetic Dentistry, Kyushu Dental College, Kitakyushu, Fukuoka, Japan, JP;(2) Department of Oral Microbiology Kyushu Dental College, 2-6-1, Manazuru, Kokura-kita, Kitakyushu, Fukuoka, 803-8580, Japan, JP |
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Abstract: | The development of the potential of osteoblasts to support bone resorption by osteoclasts in response to roughness on bone
slices was examined in the co-incubation cell system of immature osteoclasts and osteoblastic cells. The immature osteoclasts,
which need alkaline phospatase (ALP)-positive osteoblastic cells for bone resorption, were generated in mouse spleen cultures
with 1, 25-dihydroxyvitamin D3 and prostaglandin E2. ALP-negative osteoblastic cells from mouse calvaria were incubated on rough surfaced bone slices for 3 days. The number
of ALP-positive cells increased greatly on the rough surface, but little on the smooth surface. When immature osteoclasts
were added and incubated for 1 more day, the resorption pit number and the total pit areas on the smooth surface were not
much different from those before incubation but were approximately four times higher on the rough surface.
Received: 21 July 1998 / Accepted: 12 March 1999 |
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Keywords: | : Osteoclasts — Rough surface — Bone resorption — Spleen cells — Alkaline phosphatase |
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