Prenatal alcohol exposure causes the over-expression of DHAND and EHAND by increasing histone H3K14 acetylation in C57 BL/6 mice |
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| Affiliation: | 1. Heart Centre, Children''s Hospital of Chongqing Medical University, Chongqing, PR China;2. Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, PR China;3. Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, PR China;4. Chongqing Key Laboratory of Translational Medical Research in Cognitive Development and Learning and Memory Disorders, Chongqing, PR China;5. Ohio State University Medical Center, Richard Ross Heart Hospital Cardiology, Columbus, OH, USA;1. Department of Engineering Science, College of Engineering, University of Tehran, P.O. Box 11365-4563, Tehran, Islamic Republic of Iran;2. Department of Chemistry, Faculty of Sciences, Shahrekord University, P.O. Box 115, Shahrekord, Islamic Republic of Iran;3. Nanotechnology Research Center, Shahrekord University, 8818634141 Shahrekord, Islamic Republic of Iran;4. Department of Physics, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Islamic Republic of Iran;5. Department of Chemistry, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Islamic Republic of Iran;1. Flemish Institute for Technological Research (VITO NV), Environmental Risk and Health Unit, Mol, Belgium;2. Institut Català de Nanotecnologia (ICN), Barcelona, Spain;3. Università di Modena e Reggio Emilia, Laboratorio Biomateriali, Modena, Italy;4. Hasselt University, Centre for Environmental Sciences, Diepenbeek, Belgium;1. Department of Pharmacology and Neuroscience, Texas Tech University Health Sciences Center, Lubbock, TX 79430 USA;2. South Plains Alcohol and Addiction Research Center, Texas Tech University Health Sciences Center, Lubbock, TX, USA;1. Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan;2. Chemicals Evaluation and Research Institute, Japan, 1-4-25 Koraku, Bunkyo-ku, Tokyo 112-0004, Japan;3. Pathogenetic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu-shi, Gifu 501-1193, Japan;1. Institute of Physiology, Shandong Univerisity School of Medicine, 44#,Wenhua Xi Road, Jinan, Shandong, 250012 PR China;2. Department of Anesthesiology, Qilu Hospital, Shandong University, 107#, Wenhua Xi Road, Jinan, 250012 PR China |
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| Abstract: | Prenatal alcohol exposure leads to congenital heart abnormal development, its mechanisms are still unknown. Recent reports have associated alcohol exposure with histone H3 acetylation. In the present study, we have performed the experiments to test the hypothesis that histone H3K14 acetylation is the key role in the fetal heart leads to over-expression of cardiac specific genes DHAND and EHAND caused by prenatal alcohol exposure. Seventy pregnant C57BL/6 mice were divided randomly into seven groups (n = 10). They were the untreated group, dimethyl sulfoxide group, alcohol exposure group, curcumin treatment group, both alcohol and curcumin treatment group, SAHA treatment group, both alcohol and SAHA treatment group. Fetal mouse hearts were collected on embryonic day 14.5. The changes of HATs activities, the acetylation levels of histone H3K14 (H3K14ac), the expression levels of cardiac specific genes DHAND and EHAND, and structure of chromatin were determined. Our data indicates that curcumin and SAHA significantly reduces and increases the activities of HATs and the levels of histone H3K14ac in fetal hearts, respectively. The expression of DHAND and EHAND is significantly down-regulated and up-regulated in the groups treated with curcumin and SAHA. Furthermore, our results from ChIP assays have shown that the histone H3K14ac connects with the DHAND and EHAND genes are significantly inhibited by curcumin and simulated by SAHA. Our study suggests that prenatal alcohol exposure causes the over-expression of DHAND and EHAND by increasing H3K14ac in mice. |
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| Keywords: | Prenatal alcohol exposure Histone H3K14 acetylation Curcumin SAHA DHAND EHAND |
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