Ultrastructural changes in rat airway epithelium in asthmatic airway remodeling |
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| Affiliation: | 1. Pathology Department, Umraniye Education and Research Hospital, Istanbul, Turkey;2. General Surgery Department, Medeniyet University, Istanbul, Turkey;3. General Surgery Department, Umraniye Education and Research Hospital, Istanbul, Turkey;1. Department of Pathology, Samsun Training and Research Hospital, Turkey;2. Department of Pathology, 19 Mayis University, Samsun, Turkey;1. Department of Pathomorphology, Collegium Medicum, Jagiellonian University, ul. Grzegorzecka 16, 31-531 Kraków, Poland;2. Department of Urology, Collegium Medicum, Jagiellonian University, ul. Grzegorzecka 18, 31-531 Kraków, Poland;1. Laboratory of Veterinary Histopathology in Komarno, Slovak Republic;2. Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Czech Republic;3. Physiology of Nutrition and Quality of Animal Product, Institute of Animal Science in Prague – Uhrineves, Czech Republic;4. Department of Biology, Pedagogical Faculty, Selye Janos University in Komarno, Slovak Republic;5. Institute of Experimental Medicine, Academy of Sciences of the Czech Republic in Prague, Czech Republic;6. Department of Immunology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania;7. Student of Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Czech Republic;8. Department of Biology, University of Presov in Presov, Slovak Republic;1. Department of Pathology, Erasme Hospital, Brussels, Belgium;2. Department of Pathology, Universitair Ziekenhuis, Brussels, Belgium |
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| Abstract: | ObjectivesTo explore asthmatic rat airway epithelial cells mitochondrial ultrastructure changes.MethodsSix female Wistar rats of the same weight (60–80 g) were randomly divided into two groups: the asthmatic group and the control group. According to the OVA inhaled method, the asthmatic airway remodeling rat model was established. Epithelial tissue of the rat trachea was taken from the two groups for transmission electron microscopy (TEM); we counted the number of mitochondria and observed the airway ciliated epithelium, intercellular collagen deposition in the two rat groups and mitochondrial ultrastructure change.ResultsAirway multilayer ciliated epithelium develops, with cilia fallen off; goblet cells increased and irregular, mitochondrial basement membrane density is decreased, mitochondrial crista is reduced, and the nucleus has more incisures and irregular shape in asthmatic rats; airway epithelial cell matrix collagen deposition increased; and lamellar body and mitochondrial cavity formation.ConclusionsIn the asthmatic rat airway, epithelial cells undergo apoptosis and the numbers of mitochondria increased compared with the ones in normal rat airway but lose normal structure. |
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| Keywords: | Asthma Airway epithelial cell Mitochondria Ultrastructure |
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