Effects of ischemia and omeprazole preconditioning on functional recovery of isolated rat heart

Objective

The aim of this study was to compare protective effects of ischemic andpotential protective effects of pharmacological preconditioning withomeprazole on isolated rat heart subjected to ischemia/reperfusion.

Methods

The hearts of male Wistar albino rats were excised and perfused on aLangendorff apparatus. In control group (CG) after stabilization period,hearts were subjected to global ischemia (perfusion was totally stopped) for20 minutes and 30 minutes of reperfusion. Hearts of group II (IPC) weresubmitted to ischemic preconditioning lasting 5 minutes before 20 minutes ofischemia and 30 minutes of reperfusion. In third group (OPC) hearts firstunderwent preconditioning lasting 5 minutes with 100μM omeprazole, and thensubmitted 20 minutes of ischemia and 30 minutes of reperfusion.

Results

Administration of omeprazole before ischemia induction had protective effecton myocardium function recovery especially regarding to values of systolicleft ventricular pressure and dp/dt max. Also our findings are that valuesof coronary flow did not change between OPC and IPC groups in last point ofreperfusion.

Conclusion

Based on our results it seems that ischemic preconditioning could be used asfirst window of protection after ischemic injury especially because allinvestigated parameters showed continuous trend of recovery of myocardialfunction. On the other hand, preconditioning with omeprazole induced suddentrend of recovery with positive myocardium protection, although lesseffective than results obtained with ischemic preconditioning not withstand,we must consider that omeprazole may be used in many clinical circumstanceswhere direct coronary clamping for ischemic preconditioning is notpossible.