Immunohistological analysis of neurturin and its receptors in human cochlea |
| |
| Institution: | 1. Department of Pharmacology, School of Medical Sciences, Wallace Wurth Building, UNSW Australia, Sydney, NSW 2052, Australia;2. Auckland Cancer Society Research Centre, School of Medical Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand;1. Materials Center Leoben Forschung GmbH, Roseggerstrasse 12, 8700 Leoben, Austria;2. Institute of Materials Science and Technology, Vienna University of Technology, Favoritenstrasse 9-11/E308, 1040 Vienna, Austria;3. Institute of Physics of Materials, Academy of Science of the Czech Republic, ?i?kova 22, 616 62 Brno, Czech Republic;4. Institute of Mechanics, Montanuniversität Leoben, Franz-Josef-Strasse 18, 8700 Leoben, Austria;1. Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, China;2. Institute of Hearing and Speech-Language Science, Sun Yat-sen University, China;3. Zhongshan School of Medicine, Sun Yat-sen University, China;4. School of Psychology and Center for Studies of Psychological Application, South China Normal University, Guangzhou, China;1. Department of Otolaryngology-Head and Neck Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi, PR China;2. Department of Otolaryngology, Xi’an Children''s Hospital, Xi’an, Shaanxi, PR China |
| |
| Abstract: | ObjectiveDifficulties in obtaining properly preserved human cochlea have been a major obstacle to in vitro study of this deeply located and hard bone-fortressed hearing organ. Our study aimed at investigating GDNF family ligands (GFLs) and their receptors in the human cochleae that were surgically obtained during a transcochlear approach dealing with life-threatening, intra-cranial meningiomas.MethodsThe specimens were properly fixed with 4% paraformaldehyde in the operating room. By using immunohistochemical techniques, distribution of GDNF, Neurturin (NTN, one member of GFLs), as well as cRet, GFRα-1 and GFRα-2 receptors in the human cochleae was investigated. Five cochleae from five adult patients were processed for the study. The patients had normal hearing threshold before operation.ResultscRet receptor immunoreactivity was seen in the spiral ganglion neurons, mainly inside the cell bodies but rarely in the nerve fibers and not in the organ of Corti. Immunolabeling for GFRα-1 and GFRα-2 receptors was identified mainly in the cell bodies of the spiral neurons than in the nerve fibers. In the organ of Corti, GFRα-1 immunostaining could be demonstrated in the Deiters’ cells, Hensen cells, inner pillar cells, and weakly in the inner hair cells but not in the outer hair cells; no structures in the organ of Corti were labeled with GFRα-2 receptor antibody. NTN immunostaining was found in the supporting cells of organ of Corti, including Deiters’ cells, Hensen cells as well as Claudius’ cells. In the spiral ganglia, NTN immunostaining was seen in both the cell bodies and the nerve fibers of neurons. GDNF immunoreactivity was not revealed in human cochlea.ConclusionSurgically obtained human cochleae were properly fixed and underwent immunohistochemical investigation of neurotrophic elements. NTN and its receptors discovered in current study can be responsible for the unique neuronal survival properties in human spiral ganglion (hSG); a prerequisite for the function of cochlear implants. |
| |
| Keywords: | Human cochlea Spiral ganglion neuron GDNF family ligand Neurturin cRet GFRα-1 GFRα-2 |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
