A new alternative method for testing skin irritation using a human skin model: A pilot study |
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Affiliation: | 1. Dermatology Clinic, Canton Vaud University Hospital (CHUV), Lausanne, Switzerland;2. Institute of Work and Health (IST), University of Lausanne and Geneva, Lausanne, Switzerland;3. Reconstructive and Plastic Surgery Clinic, Canton Vaud University Hospital (CHUV), Lausanne, Switzerland |
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Abstract: | BackgroundStudies assessing skin irritation to chemicals have traditionally used laboratory animals; however, such methods are questionable regarding their relevance for humans. New in vitro methods have been validated, such as the reconstructed human epidermis (RHE) model (Episkin®, Epiderm®). The comparison (accuracy) with in vivo results such as the 4-h human patch test (HPT) is 76% at best (Epiderm®). There is a need to develop an in vitro method that better simulates the anatomo-pathological changes encountered in vivo.ObjectivesTo develop an in vitro method to determine skin irritation using human viable skin through histopathology, and compare the results of 4 tested substances to the main in vitro methods and in vivo animal method (Draize test).MethodologyHuman skin removed during surgery was dermatomed and mounted on an in vitro flow-through diffusion cell system. Ten chemicals with known non-irritant (heptylbutyrate, hexylsalicylate, butylmethacrylate, isoproturon, bentazon, DEHP and methylisothiazolinone (MI)) and irritant properties (folpet, 1-bromohexane and methylchloroisothiazolinone (MCI/MI)), a negative control (sodiumchloride) and a positive control (sodiumlaurylsulphate) were applied. The skin was exposed at least for 4 h. Histopathology was performed to investigate irritation signs (spongiosis, necrosis, vacuolization).ResultsWe obtained 100% accuracy with the HPT model; 75% with the RHE models and 50% with the Draize test for 4 tested substances. The coefficients of variation (CV) between our three test batches were <0.1, showing good reproducibility. Furthermore, we reported objectively histopathological irritation signs (irritation scale): strong (folpet), significant (1-bromohexane), slight (MCI/MI at 750/250 ppm) and none (isoproturon, bentazon, DEHP and MI).ConclusionsThis new in vitro test method presented effective results for the tested chemicals. It should be further validated using a greater number of substances; and tested in different laboratories in order to suitably evaluate reproducibility. |
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Keywords: | NaCl Heptylbutyrate Hexylsalicylate Butylmethacrylate Isoproturon Bentazon DEHP Methylisothiazolinone (MI) Folpet 1-Bromohexane Methylchloroisothiazolinone (MCI/MI) Sodiumlaurylsulfate Irritation scale Draize test Episkin® Epiderm® |
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