| 基于SSR分子标记的裸花紫珠种质资源遗传多样性分析及DNA指纹图谱构建 |
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| 引用本文： | 张红瑞,李鑫,陈振夏,胡文斌,李伟,黄梅,于福来,杨林立.基于SSR分子标记的裸花紫珠种质资源遗传多样性分析及DNA指纹图谱构建[J].中草药,2023,54(12):3971-3982. |
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| 作者姓名： | 张红瑞 李鑫 陈振夏 胡文斌 李伟 黄梅 于福来 杨林立 |
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| 作者单位： | 河南农业大学农学院, 河南 郑州 450046;河南农业大学农学院, 河南 郑州 450046;中国热带农业科学院热带作物品种资源研究所 农业农村部中药材生物学与栽培重点实验室/海南省热带药用植物工程研究中心, 海南 海口 571101;海南九芝堂药业有限公司, 海南 海口 570311;河南省中医院, 河南 郑州 450053 |
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| 基金项目： | 海南省重大科技计划项目（ZDKJ2021001）；海南省自然科学基金项目（322QN393） |
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| 摘 要： | 目的 研究裸花紫珠Callicarpa nudiflora种质资源遗传多样性，为其种质资源鉴定及优异种质筛选提供依据。方法 采用SSR分子标记技术，探究103份裸花紫珠种质遗传多样性，基于遗传距离进行UPGMA聚类分析，以SSR扩增条带为基础建立供试材料扩增条带DNA指纹图谱。结果 14对引物共扩增出92个等位基因（number of alleles,Na），有效等位基因（effective number of alleles,Ne）占比39.37%。平均多态性信息含量（polymorphism information content,PIC）为0.468 2,6对引物具有高度多态性（PIC>0.5),6对具有中度多态性（0.25
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| 关 键 词： | 裸花紫珠 SSR分子标记 遗传多样性 遗传距离 聚类分析 主坐标分析 DNA指纹图谱 |
| 收稿时间： | 2022/12/6 0:00:00 |
Genetic diversity analysis and DNA fingerprints construction of Callicarpa nudiflora germplasm resources based on SSR markers |
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| ZHANG Hong-rui,LI Xin,CHEN Zhen-xi,HU Wen-bin,LI Wei,HUANG Mei,YU Fu-lai,YANG Lin-li.Genetic diversity analysis and DNA fingerprints construction of Callicarpa nudiflora germplasm resources based on SSR markers[J].Chinese Traditional and Herbal Drugs,2023,54(12):3971-3982. |
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| Authors: | ZHANG Hong-rui LI Xin CHEN Zhen-xi HU Wen-bin LI Wei HUANG Mei YU Fu-lai YANG Lin-li |
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| Institution: | College of Agriculture, Henan Agricultural University, Zhengzhou 450046, China;College of Agriculture, Henan Agricultural University, Zhengzhou 450046, China;Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Cultivation of Herb Medicine(Haikou), Ministry of Agriculture and Rural Affairs/Hainan Provincial Engineering Research Center for Tropical medicinal plants, Haikou 571101, China;Hainan Jiuzhitang Pharmaceutical Co., Ltd., Haikou 570311, China; Henan Province Hospital of Traditional Chinese Medicine, Zhengzhou 450053, China |
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| Abstract: | Objective To study the genetic diversity and genetic relationship of Luohuazizhu (Callicarpa nudiflora Hook. et Arn.) germplasm resources, provide the basis for identification of germplasm resources and screening of excellent germplasm resources. Methods Genetic diversity of 103 germplasm resources was explored by SSR molecular marker technique, UPGMA cluster analysis was carried out based on genetic distance, and DNA fingerprints of amplified bands of tested materials were established based on SSR amplified bands. Results A total of 92 alleles (Na) were amplified by 14 pairs of primers, effective number of alleles (Ne) accounted for 39.37%. The average polymorphism information content (PIC) was 0.468 2, six pairs of primers were highly polymorphic (PIC>0.5) and six pairs were moderately polymorphic (0.25H) and Shannon''s information index (I) were 1.0390 and 0.5051, showing a high level of genetic diversity. Cluster analysis divided the materials into two categories:Group I included two germplasm; Group II contains 101 germplasm resources and is divided into two subclasses. Principal coordinate analysis divided the materials into three groups, which were basically consistent with the clustering results. The constructed fingerprint can distinguish germplasm by primer combination.Conclusion A total of 103 germplasms have rich genetic diversity. The DNA fingerprint of C. nudifloragermplasms was successfully constructed by 14 pairs of SSR primers. The results can provide scientific basis for germplasm identification, genetic relationship and molecular assisted breeding of C. nudifloragermplasms. |
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| Keywords: | Callicarpa nudiflora Hook et Arn SSR molecular marker genetic diversity genetic distance cluster analysis principal coordinate analysis DNA fingerprinting |
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