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| 用国产WBS-1型微波杀生器照射与断头处死对动物环核苷酸含量的影响 | |
| 引用本文： | 文元镒,刘伯春,陆纯惠,张艳萍,张淑芳,史国青. 用国产WBS-1型微波杀生器照射与断头处死对动物环核苷酸含量的影响[J]. 针刺研究, 1983, 0(2) |
| 作者姓名： | 文元镒 刘伯春 陆纯惠 张艳萍 张淑芳 史国青 |
| 作者单位： | 中国医学科学院基础医学研究所生理室，中国医学科学院基础医学研究所生理室，中国医学科学院基础医学研究所生理室，中国医学科学院基础医学研究所生理室，中国医学科学院基础医学研究所生理室，中国医学科学院基础医学研究所生理室 |
| 摘 要： | <正> 动物脑内许多重要的化学物质,如乙酰胆硷,r-氨基丁酸,肽类物质和环核苷酸等含量的高低与动物处死方法有密切关系。因此如何迅速终止脑内生化反应,准确反映动物死前瞬间化学物质的实际含量,这是神经化学研究中必须解决的一个重要问题。70年代以来,微波在生物学作用中的研究进展很快,尤其是应用微波技术来研究中枢神经系统的功能引起了特别注意。利用微波穿射动物头部时产生的热效应,可以迅速灭活脑内 |
THE EFFECT OF IRRADIATION WITH MODEL WBS-1 MICROWAVE EXPERIMENTAL ANIMAL KILLING APPARATUS AND DECAPITATION ON LEVELS OF CYCLIC NUCLEOTIDE IN ANIMAL BRAIN |
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| Abstract: | The purpose of the present work is to compare the contents of cyclic nucleotide in cerebral cortex, thalamus, hypothalamus and middle central grey of rats and rabbits by irradiation with high power model WSB-1 mi- crowave and decapitation. The results indicated that the levls of cAMP in brain areas were much higher in the decapitaion group than those in the microwave irradiation and the contents of cGMP in brain were obviou- sly decreased in decapitated animals. According to the experimental resu- lts of direct measurement of temperature and activity of phosphodiesterase in brain areas, the microwave apparatus has been proved extremely effici- ent. It is simple in construction and convenient in use. It can kill not o- nly rabbits and cats in 2 sec. but also rats and mice in 1.4-1.6 sec. It su- pplies an advanced technical means for studying neurochemistry, |
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