地西他滨联合丙戊酸钠体外诱导1例AML-M2复发患者原始细胞凋亡分化的作用研究

目的探讨地西他滨(decitabine,DCA)和丙戊酸钠(valproic acid,VPA)联用对1例复发性AML-M2患者原始细胞体外的影响。方法分选此例患者骨髓原始细胞,设立药物分组如下:对照组,DCA单药A组(1.0μmol/L),DCA单药B组(4.0μmol/L),VPA单药组(2.0mmol/L),联合用药A组(DCA 1.0μmol/L+VPA 2.0mmol/L),联合用药B组(DCA 4.0μmol/L+VPA 2.0mmol/L),作用48h。应用流式细胞术检测早期凋亡率和CD117、CD14表达率。结果相对于各自的单药组,联合用药A组和联合用药B组均能显著提高早期凋亡率和CD14表达,抑制CD117的表达(P<0.01)。结论体外DCA联合VPA能显著加强抗白血病效应。

Synergistic Effect of Decitabine and Valproic Acid on Differentiation and Apoptosis of Stem Cells of an AML-M2 Patient in vitro

Objective To investigate the synergistic effect of decitabine(DCA) and valproic acid(VPA) on differentiation and apoptosis of stem cells of an AML-M 2 patient in vitro.Methods The stem cells in the bone marrow of the AML-M 2 patient were sorted by flow cytometry.The groups were set as follows: control group;DCA alone group A(1.0μmol / L);DCA alone group B(4.0μmol / L);VPA alone group(2.0mmol / L);combination group A(DCA 1.0μmol / L + VPA 2.0mmol / L);combination group B(DCA 4.0μmol / L + VPA 2.0mmol / L).The cells were treated by drug for 48 hours.Then the apoptosis rates,CD117 and CD14 expressions were detected by flow cytometry.Results Compared with corresponding concentration single drug group,the apoptosis rates and CD14 expressions of the combination group A and B were significantly higher(P < 0.01) and CD117 expressions of the combination group A and B were significantly lower(P < 0.01).Conclusion There was an enhanced antileukemia activity of combinations of DCA and VPA.