系统进化树分析慢性丙型肝炎患者HCV基因分型

目的了解浙江省慢性丙型肝炎患者HCV基因型分布情况。方法参考Ohno的分型方法,采用巢式RT-PCR法扩增抗HCV抗体阳性患者的血清样品HCV核心(core)基因部分区段(-12nt～+343nt),克隆并测序1];以Clustalw软件对所克隆片段进行系统进化树分析并分型。结果在60份HCV抗体阳性血清中,HCV检出率为55%(33例);系统进化树分析提示共有1b、2a、3a、3b、6a 5个不同的基因型,以1b、3b和2a基因型为主,其中1b阳性率为52%(17例)。结论浙江省慢性HCV感染者中HCV基因型呈现出以1b、3b和2a为主、多基因型存在的特点。

Polygenetic Tree Analysis of HCV Genotype in Zhejiang Province

Objective To study the genotype distribution of hepatitis C virus(HCV) in Zhejiang province.Methods Fragment(-12nt-+343nt)of HCV core gene was amplified by nest RT-PCR from anti-HCV antibody positive serum and then cloned and sequenced referred to Ohno′s method.The cloned sequences were then blasted and genotyped by using Clustalw based on polygenetic tree.Results HCV RNA positive rate was 55%(33 cases) in 60 serums.Genotype 1b,2a,3a,3b and 6a existed in the 33 serum samples,among which 1b,3b and 2a genotypes were the majority.Conclusion The features of HCV infection in Zhejiang province were 1b,3b and 2a genotypes-based,multiple genotypes co-existing characteristic.