| 枸杞多酚提取物中总多酚的测定 |
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| 引用本文： | 黄丽,於洪建,吴巍,张颖.枸杞多酚提取物中总多酚的测定[J].药物评价研究,2012(4):273-275. |
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| 作者姓名： | 黄丽 於洪建 吴巍 张颖 |
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| 作者单位： | 天津中医药大学药学院;天津尖峰天然产物研究开发有限公司;辽宁植物多酚工程技术中心 |
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| 摘 要： | 目的建立紫外分光光度法测定枸杞多酚提取物中的总多酚。方法采用紫外分光光度法测定枸杞多酚提取物中总多酚,用水稀释样品,加入显色剂Filin-Ciocalteu试剂,在760 nm处测定吸光度,用同样方法测定并绘制标准曲线。结果紫外分光光度法测定枸杞多酚提取物中总多酚的量分别为24.03%、26.38%、25.45%、28.27%、29.08%,线性范围1～8μg/mL,R2=0.999 5。平均回收率为103.5%,RSD<5%。结论该方法操作简单,准确可靠,适于枸杞多酚提取物中总多酚的测定。
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| 关 键 词： | 枸杞多酚提取物 多酚 紫外分光光度计法 |
Determination of total polyphenols in polyphenol extract from Lycium barbarum |
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| Institution: | HUANG Li1,YU Hong-Jian2,3,WU Wei2,ZHANG Yin2 1.College of Pharmacy,Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China 2.Tianjin Jianfeng Natural Products R&D Co.,Ltd.,Tianjin 300457,China 3.Engineering Research Center for Research and Development of Plant Polyphenols of Liaoning Province,Shenyang 110000,China |
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| Abstract: | Objective To establish UV-spectrophotometry to determine the content of total polyphenols in polyphenol extract from Lycium barbarum(PELB).Methods The content of total polyphenols in PELB was detected by using UV-spectrophotometry.The sample was diluted with water,chromogenic agent Filin-Ciocalteu was added,the absorbance was detected at 760 nm by UV-spectrophotometry,and with the same method standard curve was measured and drawn.Results The contents of total polyphenols in different batches of PELB were 24.03%,26.38%,25.45%,28.27%,and 29.08%,respectively,the range of linearity was 1―8 μg/mL,and R2= 0.999 5.The average recovery was 103.5% and the RSD was less than 5%.Conclusion The method is proved to be simple,reliable,accurate,repeatable,and could be used to measure the content of total of polyphenols in PELB. |
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| Keywords: | polyphenol extract from Lycium barbarum(PELB) polyphenols UV-spectrophotometry |
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