晚期糖基化终产物引起内皮细胞connexin43表达上调

目的研究晚期糖基化终产物(advanced glycation end products,AGEs)对体外培养的人脐静脉内皮细胞缝隙连接蛋白(gap junction protein)connexin43基因表达的影响。方法 D-葡萄糖孵育牛血清白蛋白制备糖基化牛血清白蛋白作为晚期糖基化终产物。体外常规培养的人脐静脉内皮细胞分为4组,分别为对照组,100、200和400mg/L AGEs干预组,干预24h后通过RT-PCR方法检测各组connexin43基因mRNA的表达有无差别;通过免疫荧光方法检测对照组和200mg/L AGEs干预组之间connexin43蛋白的表达水平有无差别。结果不同浓度(100、200、400mg/L)的AGEs干预24h均能引起人脐静脉内皮细胞con-nexin43基因mRNA的表达上调(P<0.01);200mg/L的AGEs干预24h引起人脐静脉内皮细胞connexin43基因的蛋白表达上调(P<0.01)。结论 AGEs体外短期干预可引起人脐静脉内皮细胞connexin43基因的mRNA和蛋白表达水平上调。

Effect of Advanced Glycation End Products on The Expression of Connexin43 Gene in Cultured Human Umbilical Vein Endothelium Cells

Objective To investigate the effect of advanced glycation end products(AGEs) on the expression of gap junction protein Connexin43 gene in cultured human umbilical vein endothelium cells(HUVECs) in vitro.Methods Bovine serum albumin(BSA) was incubated with D-glucose to produce AGE-BSA.Human umbilical vein endothelial cells cultured in normal condition in vitro were divided into blank control group,100,200 and 400mg/L AGEs intervention groups respectively.24 hours after AGEs was given,the mRNA expression of connexin43 gene of cultured HUVECs was detected with RT-PCR method.While the protein expression level of connexin43 gene was detected and compared with immunofluorescence method between the control and 200mg/L AGEs intervention groups.Results Different concentrations(100,200,400mg/L) of AGEs all can up-regulate the mRNA expression level of connexin43 gene in cultured HUVECs after 24 hours intervention(P < 0.01).Similarly,the protein expression level of connexin43 gene of HUVECs in the group intervened with 200mg/L AGEs for 24 hours was up-regulated as compared with the control group(P<0.01).Conclusion AGEs may up-regulate the mRNA and protein expression level of connexin43 gene in cultured human vein endothelium cells within short time in vitro,indicating that connexin43 may play an important role in the pro-atherosclerotic effect of AGEs.