Ⅱ型糖尿病伴牙周炎病人牙龈组织中VEGF表达及MVD计数的研究

目的:探讨Ⅱ型糖尿病伴牙周炎病人牙龈组织中血管内皮生长因子(VEGF)的表达水平及其作用。方法:选取Ⅱ型糖尿病伴重度牙周炎(DP)病人、单纯重度慢性牙周炎(CP)病人、健康对照者(N)各15例,分别切取牙龈组织,用免疫组化染色方法检测牙龈组织中VEGF表达和MVD计数。结果:DP组牙龈组织中VEGF表达和MVD计数均显著高于CP组和N组(P<0.05);CP组高于N组(P<0.05)。结论:Ⅱ型糖尿病伴重度慢性牙周炎病人牙龈组织中VEGF表达水平和MVD计数明显升高。

VEGF expression level and MVD count in gingiva of patients with type 2 diabetes mellitus and severe chronic periodontitis

AIM:To investigate vascular endothelial growth factor(VEGF) expression level and microvessles density(MVD) count in gingival tissues of patients with type 2 diabetes mellitus and periodontitis,and to study the role of VEGF in the disease.METHODS:Patients with type 2 diabetes mellitus and severe chronic periodontitis(group DP,n =15),patients with severe chronic periodontitis(group CP,n = 15),and healthy controls without periodontal and systemic disease(group N,n=15) were included.Immunohistochemistry was used to detect the expression of VEGF and MVD count in gingival tissues of each group.RESULTS:VEGF expression and MVD count in DP group were significantly higher than those in CP and N groups(P<0.05).Furthermore,the parameters were significantly higher in CP group than in N group(P<0.05).CONCLUSION:VEGF expression level and MVD count were significantly increased in gingival tissues of type 2 diabetes mellitus with severe chronic periodontitis patient,indicating that VEGF might be used as an indicator for predicting periodontitis severity.