GuttaFlow根管充填材料对人牙周膜成纤维细胞的毒性

目的:比较GuttaFlow常温流动牙胶根管充填材料与临床常用的两种根管封闭剂AH-Plus和Endomethasone的细胞毒性。方法:制备GuttaFlow、AH-Plus和Endomethasone浸提液,并将100%原液分别稀释至50%、25%和12.5%。组织块法培养人牙周膜成纤维细胞,将上述不同材料、不同浓度的浸提液与细胞共同培养,于1、3、5、7 d镜下观察细胞形态,MTT法分别检测各组细胞增殖情况,并计算相对增殖率(RGR)以评价细胞毒性。结果:AH-Plus和GuttaFlow各浓度组在各时点的细胞OD值均与对照组无明显差异,RGR分别为77.1%～14.1%和79.8%～115.9%,毒性评级均为0～Ⅰ级,评价结果为无细胞毒性,优于Endometha-sone。结论:GuttaFlow对人牙周膜成纤维细胞无明显细胞毒性。

Cytotoxicity of GuttaFlow on human periodontal ligament fibroblasts

AIM: To compare the cytotoxicity of GuttaFlow on human periodontal ligament fibroblasts with AH-Plus and Endomethasone,two common root canal sealer.METHODS: Different concentrations of GuttaFlow,AH-Plus and Endomethasone eluates were prepared.Human periodontal ligament fibroblasts(PDLFs) were cultured by tissue culture methods with different concentrations(100%,50%,25% and 12.5%) of the above prepared eulates.The effects of each sealer on the viability of cultured cells were measured by morphological observation and MTT assay after 1,3,5 and 7days of culture.The relative growth rate(RGR) was calculated and the cytotoxicity was graded.RESULTS: The RGR of GuttaFlow at different time points was 79.8%-115.9%,similar to the value of 77.1%-141% of AH-plus group,indicating a cytotoxicity scale of 0-I.However,although the RGR of Endomethasone group was comparable to the other two groups on day 1,the RGR was 13.6%,43.5%,and 60.7% respectively for 100%,50% and 25% Endomethasone eluates on day 7,showing a cytotoxicity scale of II-IV.CONCLUSION: GuttaFlow,like AH-plus,showed no obvious cytotoxicity to PDLFs.