ADVIA2120血液分析仪对动物血细胞的分析性能评价

目的对ADVIA2120血细胞分析仪的动物血细胞分析性能进行评价。方法对该仪器的准确度、精密度、线性和携带污染率进行评价,并与Hemavet 950FS动物血细胞分析仪测定结果及人工白细胞分类镜检结果进行比较。结果该仪器的准确度、批内和批间精密度、携带污染率和线性均在允许范围内;与Hemavet 950FS动物血细胞分析仪测定结果相关性白细胞(WBC)、红细胞(RBC)、血红蛋白(HGB)、血小板(PLT)较好,红细胞压积(HCT)较差;白细胞分类测定结果与人工显微镜分类比较,中性粒细胞、淋巴细胞、嗜酸粒细胞相关性较好,单核细胞、嗜碱粒细胞相关性较差。结论 ADVIA2120全自动血细胞分析仪在测定动物全血样本时准确度、精密度、线性较好,携带污染率低,有较强的筛选能力,是一台性能良好的全自动血细胞分析仪,可以满足药物安全性评价课题动物血液样本分析的需要。

Evaluation of ADVIA 2120 Hematology Analyzer on blood cell of animals

Objective To evaluate the performance of ADVIA 2120 Hematology Analyzer on blood cell analysis of common laboratory animals.Methods The blood samples were analysed by ADVIA 2120 Hematology Analyzer in terms of accuracy,precision,linearity,and the contamination rate.The results were compared with those of Hemavet 950FS Blood Cell Counter as well as manual blood smears under microscopy.Results The values of the accuracy,precision,linearity,and the contamination rate were all below the qualified standardization.ADVIA 2120 had a good correlation with Hemavet 950FS in WBC,RBC,HGB,and PLT.It also correlated very well with the manual blood smears under microscopy such as neutrophil,lymphocyte,and eosinophil,although some differences were observed in the results of monocyte and basophil.Conclusion The performance of ADVIA 2120 is good and reliable.It could satisfy the hematological analysis of drug evaluation studies.