e抗原阳性的乙型肝炎患者HBV-DNA载量与HBsAg以及肝功能关系的研究

目的探讨e抗原(HBeAg)阳性的乙型肝炎患者血清中HBV-DNA载量与乙型肝炎病毒表面抗原(HBsAg)、丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)水平的相关性。方法收集319例HBeAg阳性的乙肝患者血清,用实时荧光定量PCR法检测HBV-DNA,用时间分辨免疫荧光法检测HBsAg水平,使用全自动生化仪检测ALT和AST。结果Spearman相关分析显示,HBV-DNA载量与HBsAg水平具有显著相关性(r=0.514,P<0.001);与ALT、AST水平无显著相关性;ALT水平正常组的HBV-DNA载量与HBsAg水平的相关系数更佳(r=0.535 vs r=0.514)。结论 HBeAg阳性乙型肝炎患者血清中HBV-DNA载量与HBsAg水平具有显著相关性。

Relationship Between HBV-DNA and HBsAg,Aspartate Aminotransferase and Alanine Aminotransferase in Chronic Hepatitis B Patients with Positive e Antigen

Objective To study the relationship between hepatitis B virus DNA(HBV-DNA) and HBsAg,alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in chronic hepatitis B patients with positive e antigen.Methods HBV-DNA,HBsAg,ALT and AST levels of 319 chronic hepatitis B patients with positive e antigen were detected by realtime-PCR,time-resolved fluoroimmunoassay(TRFIA) and auto biochemistry analyzer respectively.The data was analyzed by SPSS software.Results There was some correlation(r=0.514,P<0.001) between HBV-DNA and HBsAg levels,while there was no correlation between HBV-DNA and ALT levels.In the patients with normal ALT levels,the correlation between HBV-DNA and HBsAg was much better(r=0.535,P<0.001).Conclusion There was correlation between HBV-DNA and HBsAg levels in chronic hepatitis B patients with positive e antigen.