| 淡豆豉药材的高效液相指纹图谱研究 |
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| 引用本文： | 廖丽娜,张明敏,曹尉尉,毛峻琴. 淡豆豉药材的高效液相指纹图谱研究[J]. 药学实践杂志, 2012, 30(5): 351-352,386 |
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| 作者姓名： | 廖丽娜 张明敏 曹尉尉 毛峻琴 |
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| 作者单位： | 安徽中医学院;解放军第411医院;解放军第85医院 |
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| 基金项目： | 上海市卫生局项目(2010Y006A) |
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| 摘 要： | 目的建立淡豆豉药材高效液相色谱指纹图谱(HPLC-FPS),为其质量控制提供依据。方法应用高效液相色谱法(HPLC),利用反相色谱柱:Diamonsil C18(250 mm×4.6 mm,5μm),流动相:乙腈-3%冰乙酸梯度洗脱,流速:1 ml/min,柱温:30℃,检测波长:261 nm,进样量:10μl。结果不同产地淡豆豉在化学成分方面具有明显相似性,可建立淡豆豉的指纹图谱。结论建立的HPLC指纹图谱可用于淡豆豉的质量评价。
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| 关 键 词： | 淡豆豉 高效液相色谱法 高效液相色谱指纹图谱 |
| 收稿时间： | 2012-03-04 |
| 修稿时间： | 2012-05-09 |
Study on HPLC fingerprint of Semen Sojae Preparatum(SSP) |
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| LIAO Li-n,ZHANG Ming-min,CAO Wei-wei and MAO Jun-qin. Study on HPLC fingerprint of Semen Sojae Preparatum(SSP)[J]. The Journal of Pharmaceutical Practice, 2012, 30(5): 351-352,386 |
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| Authors: | LIAO Li-n ZHANG Ming-min CAO Wei-wei MAO Jun-qin |
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| Affiliation: | 1.Anhui Institute of Traditional Chinese Medicine,Hefei 230038,China;2.411st hospital of PLA,Shanghai 200433,China;3.85th hospital of PLA,Shanghai 200053,China) |
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| Abstract: | Objective To establish a HPLC fingerprint method for Semen Sojae Preparatumto quality control.Methods HPLC analysis was performed on a Diamonsil C18 column(250 mm×4.6 mm,5 μm),the mobile phase was used in gradient elution consisted of methanol-water(3% acetic acid),the flow rate was 1.0 ml /min,column temperature was 30℃ and UV detected was set at 261 nm,the sample weight:10 μl.Results The compound composition of different SSP origins was obviously comparable,which could be determined by HPLC fingerprint of the SSP.Conclusion This method could be utilized as a quality control measurement for SSP. |
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| Keywords: | SSP high performance liquid chromatography(HPLC) high performance liquid chromatographic fingerprint(HPLC-FPS) |
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