金银花多酚类物质的纯化研究

目的考察常用的大孔吸附树脂对金银花多酚类物质的纯化效果,筛选纯化工艺条件。方法以总酚的质量分数和回收率作为指标,采用静态吸附及动态吸附考察树脂的吸附、解吸性能。结果 NKA-2型树脂对多酚类物质的吸附能力明显强于其他树脂,其载药量为7倍树脂体积,水洗5倍树脂体积,60%乙醇洗6倍树脂体积。此条件下,浸膏量减少40%左右,总酚质量分数提升近一倍,达到60%左右,样品回收率达到55%。结论 NKA-2型树脂对金银花多酚类的纯化效果较好,适用于金银花多酚类物质的分离纯化。

Purification of polyphenols from Lonicerae Flos

Objective To study the process parameters for purification of polyphenols from Lonicerae Flos by commonly used macroporous resins,and to optimize the purification technological conditions.Methods The purity and recovery of polyphenols were taken as indexes,and the adsorption and desorption performance by difference resins were studied by using static adsorption and dynamic adsorption.Results It has been found that NKA-2 resin’s adsorption capacity of polyphenols was the best among those resins.The optimum condition is seven volume times of sample,five volume times of water washing,and six volume times of 60% ethanol washing.Under those conditions,40% extract was reduced with the purity of polyphenols around 60%,and the recovery rate was 55%.Conclusion NKA-2 resin has a good performance and is suitable for the production of polyphenols from Lonicerae Flos.