HPLC法同时测定双黄连制剂中绿原酸、连翘苷、黄芩苷

目的建立同时测定双黄连制剂中绿原酸、连翘苷和黄芩苷的HPLC方法。方法色谱柱为Agilent ZORBAXXDB C18柱(250mm×4.6mm,5μm);流动相为甲醇-0.2%磷酸溶液,线性梯度洗脱,体积流量为1.0mL/min,检测波长为324、280nm,柱温30℃。结果绿原酸、连翘苷、黄芩苷的线性范围分别为0.093～1.860、0.022～0.440、0.328～6.560μg,平均加样回收率分别为98.8%、98.4%、99.3%。结论该方法简便、快速、准确,可作为双黄连制剂的质量控制方法。

Simultaneous determination of chlorogenic acid,forsythin,and baicalin in Shuanghuanglian preparations by dual-wavelength HPLC

Objective To establish an HPLC method for simultaneously determining chlorogenic acid,forsythin,and baicalin in Shuanghuanglian preparations.Methods HPLC assay was carried out using an Agilent ZORBAX XDB C18 column with column temperature at 30℃;The mobile phase was methanol-0.2% phosphoric acid solution as gradient elution with flowing rate of 1.0 mL/min;And the detection wavelengths were 324 and 280 nm.Results The linear ranges of chlorogenic acid,forsythin,and baicalin were 0.093—1.860,0.022—0.440,and 0.328—6.560 μg respectively,and the average recoveries of three components were 98.8%,98.4%,and 99.3% respectively.Conclusion The method is simple,quick,and accurate,and may be used for the quality control of Shuanghuanglian preparations.