RUNX3基因甲基化与鼻咽癌的临床生物学行为关系

目的探讨RUNX3基因甲基化与鼻咽癌临床生物学行为关系。方法运用甲基化特异性PCR对54例鼻咽癌组织、18例慢性鼻咽炎症组织和20例正常鼻咽上皮组织的RUNX3基因启动子区甲基化状态进行检测。结果 RUNX3基因在鼻咽癌组织中启动子甲基化频率为59%(32/54);而在慢性鼻咽炎症组织和正常鼻咽上皮组织中均未检测到RUNX3基因启动子甲基化。RUNX3基因启动子区甲基化与患者临床生物学行为无明显相关关系。结论 RUNX3基因甲基化具有肿瘤特异性,RUNX3基因甲基化参与鼻咽癌发生发展,但目前尚不能作为判断鼻咽癌临床预后预测指标。

Relationship Between Methylation of RUNX3 Gene and Biological Behavior of Nasopharyngeal Carcinoma

Objective To assess the relations between methylation of RUNX3 gene and the biological behavior of nasopharyngeal carcinoma.Methods The methylation-specific PCR(MSP) was used to detect methylation level of RUNX3 tumor suppressor gene in 54 cases of nasopharyngeal carcinoma and 18 cases of chronic nasopharyngitic and 20 cases of normal nasopharyngeal epithelia tissues.Results The methylation expression rates of RUNX3 gene in nasopharyngeal carcinoma tissues were 59%(32/54),while there were no methylation in 18 cases of chronic nasopharyngitic and 20 cases of normal nasopharyngeal epithelia.It has no close correlation between methylation of RUNX3 gene and the biological behavior of nasopharyngeal carcinoma.Conclusion Methylation of RUNX3 gene has high specificity in distinguishing cancers from normal tissues.It is closely associated with the tumorigenesis and development of nasopharyngeal carcinoma,but it may not be used as an parameter in evaluating the clinical prognosis of nasopharyngeal carcinoma.