产ESBLs大肠杆菌感染大鼠实验模型的建立与评价

目的建立产超广谱β-内酰胺酶(ESBLs)大肠杆菌感染的SD大鼠败血症实验模型,为研究耐药菌感染的发病机制及药物治疗提供基础。方法采用尾静脉注射法对SD大鼠进行产ESBLs大肠杆菌感染,确定半数致死浓度,观察感染后大鼠一般状态、血培养、白细胞计数、病理切片观察重要脏器病理改变。结果模型组大鼠均出现不同程度的感染症状,血培养阳性,体温升高、体重下降、白细胞升高,与空白对照组相比,差异具有统计学意义(P<0.05),病理切片发现模型组各脏器出现炎症、细胞水肿和淤血等病理变化。结论表明了SD大鼠对产ESBLs大肠杆菌易感,并产生以体温升高、体重下降、白细胞升高,各重要脏器炎性病理改变为特征的感染表现。

Establishment and Evaluation on Infected Rats Model of ESBLs-producing Escherichia Coli

Objective To establish ESBLs-producing Escherichia coli infected SD rats model and make a theoretical basis for the study of the pathogenesis of drug-resistant bacterial infection and drug treatment.Methods The SD rats was infected with ESBLs producing E.coli by injecting into tail vein to produce infected animal,determined the media lethal dose,observed and noted common state,grown bacterial cultures,taken count of leucocyte and pathological changes of vital organs.Results The infected animal appeared varying degrees symptoms of infectionrats,such as fever,weight loss and leukocytosis.The result of bacterial cultures was positive,and as compared with the control group,the difference was statistically significant(P<0.05).The pathology found that the organ of model group animals appeared inflammation,cell edema and congestion and other pathological changes.Conclusion The SD rats was susceptible to ESBLs producing E.coli,and appeared performance of infection such as fever,weight loss,leukocytosis,inflammatory pathological changes in vital organs after infection.