SDF-1/CXCR4的表达与喉癌组织VEGF-C表达、淋巴管生成及预后的相关性

目的探讨趋化因子SDF-1及其受体CXCR4在喉癌组织中的表达与喉癌中VEGF-C表达、淋巴管生成及预后的相关性。方法应用免疫组化法检测SDF-1、CXCR4和VEGF-C在45例喉癌和20例声带息肉组织中的表达,应用5’-核苷酸酶染色法(5’-Nase)计数淋巴管密度,并结合临床病理特征进行相关分析,应用Kaplan-Meier法评估SDF-1和CXCR4表达对喉癌预后的影响。结果喉癌组织中SDF-1、CXCR4和VEGF-C表达较声带息肉组显著增加(P<0.05),SDF-1、CXCR4表达与TNM分期、淋巴结转移、淋巴管浸润相关(P<0.05),VEGF-C表达与淋巴结转移、淋巴管浸润相关(P<0.05)。SDF-1、CXCR4和VEGF-C表达呈正相关(P<0.05),三者均与瘤内和瘤周淋巴管密度相关(P<0.05)。SDF-1阳性表达生存率显著低于阴性表达(P<0.05)。结论 SDF-1/CXCR4生物轴可能通过上调VEGF-C表达促进喉癌淋巴管生成和淋巴结转移,SDF-1高表达是喉癌预后不良的重要指标。

Correlation between SDF-1,CXCR4 and VEGF-C,Lymphangiogenesis,Prognosis in Laryngeal Carcinoma

Objective To investigate the relationship between the expression of SDF-1,CXCR4 and VEGF-C,lymphangiogenesis,prognosis significance in patients with laryngeal cancer.Methods The expressions of SDF-1,CXCR4 and VEGF-C in 45 specimens of the laryngeal cancer and 20 specimens of polyp of vocal cord tissues were observed by immunohistochemistry.Lymphatic microvessed density(LVD) was evaluated by 5′-Nase staining,and the correlation analysis was made between SDF-1,CXCR4 and VEGF-C,clinical characteristics,and judged SDF-1 and CXCR4 roles in laryngeal cancer prognosis by using Kaplan-Meier method.Results The expression of SDF-1,CXCR4,VEGF-C protein were higher in laryngeal cancer tissue than in the polyp of vocal cord tissue.The expression of SDF-1 and CXCR4 were significantly correlated with TNM stage,lymph node metastasis,lymphovascular invasion.VEGF-C was correlated with lymph node metastasis,lymphovascular invasion.The expression of the SDF-1,CXCR4 and VEGF-C protein in laryngeal cancer has directly correlation.SDF-1,CXCR4,VEGF-C were significantly correlated with peritumoral and intratumoral LVD.The expression of SDF-1 had effect on survival ratio.Conclusion SDF-1/CXCR4 axis might stimulate VEGF-C upregulation and promote lymphangiogenesis,lymphatic metastasis in laryngeal cell carcinoma.The expression of SDF-1 may be helpful for judging prognosis in laryngeal cancer.