不同提取工艺的丹参提取物在大鼠体内的药动学研究

目的研究不同提取方法所得丹参提取物中丹参酮ⅡA与丹酚酸B在大鼠体内的药动学。方法大鼠禁食12h后,分别将加热回流法、集成法、醇酸回流法和梯度渗漉法所得丹参提取物ig给予大鼠,对不同时刻血浆中的目标成分进行定量测定,绘制出各提取方法下的药时曲线并计算药动学参数。结果对于丹参酮ⅡA而言,加热回流法、集成法、梯度渗漉法和醇酸回流法所得提取物大鼠体内的Cmax分别为（0.25±0.03）、（0.35±0.06）、（0.32±0.04）、（0.24±0.02）mg/L;AUC为（101.97±12.95）、（130.46±18.83）、（128.67±16.63）、（100.11±13.76）mg.min/L。对于丹酚酸B而言,以上四种方法所得提取物在大鼠体内的Cmax分别为（7.17±0.97）、（11.98±1.75）、（10.24±1.05）、（12.16±2.08）mg/L;AUC为（697.31±80.32）、（833.41±96.53）、（719.39±102.41）、（906.96±125.87）mg.min/L。结论对定量测定和药动学参数进行统计分析,可知集成法所提取的丹参脂溶性成分和水溶性成分含量均比较高,并在体内有较好的吸收,说明该法所得的提取物中丹参酮ⅡA和丹酚酸B的质量分数较高,提取效果可能较好。

Pharmacokinetics of extract from Salvia miltiorrhiza by different methods in rats

Objective To study the pharmacokinetics of tanshinone II and salvianolic acid B extracted from Salvia miltiorrhiza by different methods in rats.Methods After fasted for 12 h,extracts from S.miltiorrhiza by different methods,such as heating reflux method,integrated method,gradient percolation,and acidity alcohol reflux method,were given to rats by ig administration,respectively.HPLC was used to determine the plasma concentration of active ingredients at different time points,then the concentration-time curve was mapped out and the pharmacokinetic parameters were calculated.Results For tanshinoneⅡA,the main pharmacokinetic parameters of heating reflux,integrated method,gradient percolation,and acidity alcohol reflux method were as follows: Cmaxwere(0.25 ± 0.02),(0.35 ± 0.05),(0.32 ± 0.04),and(0.24 ± 0.02) mg/L;AUC were(101.97 ± 12.95),(130.46 ± 18.83),(128.67 ± 16.63),and(100.11 ± 13.76)(mg.min)/L.For salvianolic acid B,the main pharmacokinetic parameters of different extracting methods were as follows: Cmaxwere(7.17 ± 0.97),(11.98 ± 1.75),(10.24 ± 1.05),(12.16 ± 2.08) mg/L;AUC were(697.31 ± 80.32),(833.41 ± 96.53),(719.39 ± 102.41),and(906.96 ± 125.87) mg.min/L.Conclusion The determination and statistical analysis of pharmacokinetic parameters demonstrated that compared with other methods,the extract of integrated method has higher content of liposoluble and water-soluble constituents,and good absorption in vivo,which means this method has better effect and the contents of tanshinone II and salvianolic acid B are higher.