自动采样技术研究梓醇的药动学

目的应用自动采样技术和传统采样方法对梓醇在大鼠体内的药动学进行研究,比较两种采样方法对梓醇药动学参数的影响。方法成年Wistar大鼠12只,随机分为2组,ig给予大鼠100mg/kg梓醇供试品后不同时间点分别采用自动采样技术与传统手动采样方法收集大鼠血样,经LC-MS/MS方法分析测得不同时间的血药浓度,应用DAS2.0计算程序、非隔室模型计算药动学参数并以单因素方差分析法对两种方法所得的药动学参数进行评价。结果大鼠ig给予梓醇供试品100mg/kg后,两组试验的血药浓度数据的变化趋势高度一致,两条平均药-时曲线趋势接近,达峰时间分别为1.5、1.0h,Cmax相差10%左右,计算所得各药动学参数单因素方差分析结果显示两组的各个参数均无显著差异。结论采用两种方法收集的血样分析计算所得的梓醇在大鼠体内药动学特征基本相同。

Pharmacokinetics of catalpol by automated sampling

Objective To study the pharmacokinetics of catalpol in rats by two different blood sampling techniques(manual sampling vs automated sampling) and compare the two methods on pharmacokinetics of catalpol.Methods Two different blood sampling techniques were used in two groups of rats after ig administration of 100 mg/kg catalpol.The plasma concentration of catalpol was determinated by LC-MS/MS method.The main pharmacokinetic parameters were obtained by DAS 2.0 program and evaluated by Oneway ANOVA.Results After ig administration of 100 mg/kg catalpol to the rats,the concentration-time curves of both sampling techniques were similar,and the tmax were 1.5 and 1.0 h.Cmax of automated sampling group was 10% higher than that of the manual sampling group.Conclution The pharmacokicetic parameters of two groups are basically the same.