急性坏死性胰腺炎大鼠硫氧还蛋白-1肺表达的实验研究

目的：探讨内源性硫氧还蛋白一1（Thioredoxin-1，Trx-1）在急性坏死性胰腺炎（AcuteNecrotizingPancreatitis，ANP）大鼠模型肺组织中的表达。方法：将48只SD大鼠随机分成对照组（C组，n=24）、急性胰腺炎组（A组，n=24）。A组用6％的左旋精氨酸（L-Arginine，L—Arg）腹腔内注射，每次1．5mg／g体重，共3次，注射间隔1h，诱导ANP模型；C组同法注射等量生理盐水。在注射完L—Arg后的第6小时、第12小时和第24小时3个时点分批处死大鼠，应用免疫组织化学技术检测各组ANP肺组织Trx-1的表达，并观察各组对应各时点胰腺和肺组织病理学改变的变化。结果：①A组大鼠胰腺和肺组织病理损伤在各时点比C组明显加重（P〈O．01）；②A组肺组织Trx-1各时点表达较C组明显增强（Pd0．01）；③在第24小时，A组血清淀粉酶较C组显著升高（Pd0．01）；④A组胰腺病理损伤与血清淀粉酶、肺损伤评分、肺免疫组化评分呈正相关（rx=0．374，P=0．046；rx=0．452，P=0．027；n=0．500，P=0．013）。结论：在ANP肺组织损伤程度与胰腺组织损伤程度密切相关。在ANP肺损伤组织中Trx-1表达显著增加，肺组织Trx-1过度表达与急性胰腺炎相关性肺损伤关系密切。

EXPRESSION OF TRX-1 IN RAT LUNGS WITH ACUTE NECROTIZING PANCREATITIS

Objective：To study the expression of thioredoxin-l（Trx-1） in rat lungs with L-arginine（L-Arg）- induced acute necrotizing pancreatitis（ANP）. Methods：Forty-eight Spraque-Dawley male rats were randomly divided into groups A and C. ANP was induced by injected intraperitoneally three times with 1. 5 mg/g body weight 6~ L-Arg in an interval of 1 hour in group A. Group C received saline alone with the samemethods. Rats were killed at the 6th,12th and 24th hours after the last time of L-Arg injection. The expression of Trx-1 in the lung tissues was detected with the method of immunohistochemistry. The patho- logical changes of pancreatic and lung tissues were respectively analyzed and scored according to Kusser＇s and Lei weizhang＇s standard. Results：OWith time going,the pathological injuries of the pancreas and lungs in group A were more serious than those in group C （ P 〈0.01）. （1）The expression of Trx-1 in lungs in group C was significantly lower than that in group A （ P 〈0.01）. （3）At the 24th hour,the serum amylase in group A was significantly higher than that in group C （ P 〈0.01）. （4）Pathological injury score in pancreas was correlated with serum amylase, the lung injury and the lung immunohistochemistry score in Group A （ rs =0. 374, P =0. 0463 rs =0. 452, P =0. 027; rs =0. 500, P =0. 013）. Conclusion：Lung injury is closely related to pancreatic tissue injury in ANP. The expression of Trx-1 in the lung injury increases significantly in ANP. Overexpression of Trx-1 in lung tissues associates closely with acute pancreatitis-associated lung injury.