| PAGE一步性低背景CBBR_（250）染色方法的探讨 |
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| 引用本文： | 王太重,李栋,樊秀华,王学东,廖声荣. PAGE一步性低背景CBBR_（250）染色方法的探讨[J]. 武汉大学学报(医学版), 1995, 0(1) |
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| 作者姓名： | 王太重 李栋 樊秀华 王学东 廖声荣 |
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| 作者单位： | 武汉附一医院检验科 |
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| 摘 要： | 传统的考马斯亮蓝Ｒ２５０（ＣＢＢＲ２５０）染色方法存在背景深，耗时长，试剂耗用多的缺点，考马斯亮蓝Ｇ２５０（ＣＢＢＧ２５０）虽然需时短，但其灵敏度效低。本文探讨了一步性低背景ＣＢＢＲ２５０染色方法在蛋白质ＰＡＧＥ中的应用，结果表明使用乙醇－乙酸－水染色系统，ＣＢＢＲ２５０的最佳工作浓度为０．００４‰～０．００８‰，染色时间为８～１０ｈ，灵敏度为０．５μｇ／带。一步性ＣＢＢＲ２５０染色方法与传统的ＣＢＢＲ２５０染色方法相比，有需时短，背景浅，试剂用量少的优点，可用于蛋白质的定性和定量研究。
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| 关 键 词： | 考马斯亮蓝R_（250）；聚丙烯酰胺凝胶电泳；染色方法 |
A Study on the Method of One Step,Low Background Protein Staining with CBBR_(250) in PAGE |
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| Wang Taizhong,Li Dong,Fan Xiuhua,et al. A Study on the Method of One Step,Low Background Protein Staining with CBBR_(250) in PAGE[J]. Medical Journal of Wuhan University, 1995, 0(1) |
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| Authors: | Wang Taizhong Li Dong Fan Xiuhua et al |
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| Abstract: | This paper studied a new method for protein staining with CBBR_250 in PAGE which had shallower background,and needed less time and reagents than the traditional method.The result showed that in the staining system with alcohol-acetic acid-H20 as solvent,the optimal working concentration of CBBR250 was from 0.004‰ to 0.008‰,the recommended staining time was 8～10 hours,and the sensitivity was 0.5μg protein per electrophoresis zone. |
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| Keywords: | coomassie brilliant blue electrophoresis polyacrylamide staining method |
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