| 杜氏盐藻核基质的制备 |
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| 引用本文： | 王天云,柴玉荣,侯卫红,谢华,赵青赞,王宁,薛乐勋.杜氏盐藻核基质的制备[J].郑州大学学报(医学版),2004,39(1):39-41. |
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| 作者姓名： | 王天云 柴玉荣 侯卫红 谢华 赵青赞 王宁 薛乐勋 |
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| 作者单位： | 1. 郑州大学细胞生物学研究室,郑州,450052;新乡医学院细胞生物学教研室,新乡,453003
2. 郑州大学细胞生物学研究室,郑州,450052 |
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| 基金项目： | 国家自然科学基金资助项目 3 0 2 70 0 3 1，国家高技术研究发展 ( 863 )计划 2 0 0 2AA62 80 5 0 |
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| 摘 要： | 目的：制备盐藻核基质，进而分离核基质结合区(matrix attachment regions)。方法：采用体积分数0．5％ TritonX—100破碎细胞，体积分数15％Percoll分离盐藻细胞核，二碘水杨酸锂(1ithium diiodosalicylate，LIS)抽提核蛋白，SDS—PAGE电泳分析蛋白质成分。结果：分离出了高纯度的盐藻细胞核，电泳分析表明绝大部分核蛋白已被去除。结论：体积分数0．5％TritonX—100能有效破碎盐藻细胞，体积分数15％Percoll可分离高纯度的盐藻细胞核，25mmol／L LIS抽提可除去盐藻绝大部分核蛋白获得核基质。
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| 关 键 词： | 盐藻 核基质 核基质结合区 SDS—PAGE |
| 修稿时间： | 2003年10月25 |
Preparation of nuclear matrix of Dunaliella salina |
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| WANG Tianyun ,CHAI Yurong ,HOU Weihong ,XIE Hua ,ZHAO Qingzhan ,WANG Ning ,XUE Lexun Laboratory for Cell Biology,Zhengzhou University,Zhengzhou.Preparation of nuclear matrix of Dunaliella salina[J].Journal of Zhengzhou University: Med Sci,2004,39(1):39-41. |
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| Authors: | WANG Tianyun CHAI Yurong HOU Weihong XIE Hua ZHAO Qingzhan WANG Ning XUE Lexun Laboratory for Cell Biology Zhengzhou University Zhengzhou |
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| Institution: | WANG Tianyun 1,2),CHAI Yurong 1),HOU Weihong 1),XIE Hua 1),ZHAO Qingzhan 1),WANG Ning 1),XUE Lexun 1) 1)Laboratory for Cell Biology,Zhengzhou University,Zhengzhou 450052 2)Department of Cell Biology,Xinxiang Medical College,Xinxiang 453003 |
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| Abstract: | Aim: Through preparing nuclear matrix of Dunaliella salina, in order to isolate the matrix attachment regions further.Methods: The D.salina cells were disrupted with 0.5%TritonX-100 and purified by centrifugation using 15% Percoll.The nuclear proteins were removed using 25 mmol/L lithium diiodosalicylate and the component of protein was analyzed by SDS-PAGE. Results: The pure nuclei of D.salina were isolated, and most protein component of nuclear protein was removed. Conclusion: The matrix of D.salina may be prepared through 25mmol/L LIS extracting nuclear protein. |
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| Keywords: | Dunaliella salina nuclear matrix matrix attachment region SDS-PAGE |
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