芦荟原生质体的电融合研究

目的:探讨芦荟原生质体电融合条件,为芦荟种质改良提供细胞工程技术。方法:以芦荟无菌苗为材料,用化学酶解法处理芦荟幼叶得到原生质体,并且进行电融合。结果:在交流电电场强度(E)1.60×10~5 V/m作用时间20 s,直流脉冲强度(E)0.90×10~6 V/m、脉冲宽幅30μs,施加1次脉冲后,可使细胞有效融合率达到17.78%。结论:芦荟原生质体经过一定电场刺激,可实现原生质体的融合,供抗感染药用植物资源的种质改良提供参考。

Studies on the electrofusion of protoplasts from Aloe vera L.

Objective： To explore the appropriate conditions of Aloe vera L. Protoplasts electrofusion, for improvement of Aloe germplasm resource. Methods： The protoplast was got from Aloe leaves from tissue cultured plantlet by the emzymatic treatment and then electrofusion of those protoplasts was achieved. Results： The effective fusion rate reached 17.78% when the isolated protoplasts were placed under AC field （1.60 × 10^5 V/m, 1 MHz） for 20 s and then DC field （0.90 × 10^6 V/m） for 30 μs. Conclusion： Aloe protoplasts could be obtained by the enzymatic digesting. The given electric field could stimulate the cell-cell fusion of the protoplasts. The method provides cell engineering technique for the improvement of Aloe gempalsm resource of anti-infectious potential.