薄层扫描法测定肾宁颗粒中盐酸水苏碱的含量

目的:建立以薄层扫描法测定肾宁颗粒中盐酸水苏碱含量的方法。方法:展开剂为丙酮-无水乙醇-盐酸(10∶6∶1) ,显色剂为碘化铋钾试液-1%三氯化铁无水乙醇溶液(2∶1) ,测定波长为530nm ,参比波长为670nm ,狭缝大小为0.4mm×0. 4mm ,线性化参数Sx=3。结果:盐酸水苏碱点样量在2.412μg～6.432μg范围内斑点峰面积积分值呈良好的线形关系(r=0.9972) ,平均回收率为98.93% (RSD=1.15 % )。结论:本方法简便、准确、重现性好,可用于肾宁颗粒的质量控制

Determination of Contents of Hydrochloric Stachydrine in the Shenning Granule by TLC-scanning Method

OBJECTIVE:To determine the contents of hydrochloric stachydrine in the shenning granule by TLC-scanning Method.METHODS:The acetone-dehydrated alcohol-HCl(10∶6∶1)were used as developing agents,the potassium hep-taiodobismuthate test solution-1%trichloride ferric dehydrated alchhol solution(2∶1)were used as the color-developing a-gents,the detection wavelength was530nm,the reference wavelength was670nm,the slit size was0.4mm×0.4mm,the lin-earization parameter Sx=3.RESULTS:The spot peak area score assumed a satisfactory linear relationship when the electric sample size for the hydrochloric stachydrine at a range of2.412μg～6.432μg(r=0.9972),the recovery was98.93%(RSD=1.15%).CONCLUSION:This method is simple,accurate,and this determination method has a good reproducibility,which can be used for the quality control of the shenning granule.