Possible involvement of protein kinase C (PKC) in the regulation of electrically evoked serotonin (5-HT) release from rabbit hippocampal slices |
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| Authors: | T J Feuerstein C Allgaier G Hertting |
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| Institution: | Institut für Pharmakologie und Toxikologie, Universit?t Freiburg, F.R.G. |
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| Abstract: | Protein kinase C (PKC)-activating phorbol esters enhanced the electrically evoked 5-HT release from rabbit hippocampal slices preincubated with 3H]5-HT. The release was diminished by polymyxin B, an inhibitor of PKC. These results are compatible with a stimulatory effect of PKC on the 5-HT release induced by action potentials. The mutual effects of PKC affecting drugs on 5-HT release suggest a functional but not a competitive interaction. The attenuation or the enhancement of effects of 5-HT autoreceptor ligands at various 5-HT biophase concentrations found after PKC-affecting drugs are in line with the view that autoreceptor-mediated events are not directly influenced by the enzyme PKC. |
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