人类白细胞抗原—DRB1和DQB1基因与肺结核合并糖尿病的相关性分析

目的 探讨人类白细胞抗原（HLA）－DRB1手DQB1基因与肺结核合并2型糖尿病的关联性；寻找与肺结核合并2型糖尿病可能相关的HLA基因。方法 采用病例对照和聚合酶链反应－特异性序列引物（PCR－SSP)方法，对我国北方汉族123例肺结核合并2型糖尿病患者和与其无因缘关系的46名健康对照以及45全单纯2型糖尿病患者分别进行HLA－DRB1和DQB1闰点的等位基因分型。结果 肺结核合并2型糖尿病患者组中DRB1＊09基因频率明显高于健康人组，分别为25．10％和14．03％，RR为2．22，肺结核合并2型糖尿病患者组中DRB1＊09基因频率明显高于单纯2型糖尿病患者组，分别为25．10％和9．32％，RR为3．16，统计学上差异均有显著性；在肺结核合并Ⅱ型糖尿病患者组中DQB1＊05基因频率明显低于单纯2型糖尿病患者组，分别为7．17％和21．12％，RR为0．26，统计学差异有非常显著性。结论 研究提示DRB1＊09基因可能是肺结核合并2型糖尿病的易感基因；DQB1＊05基因可能是肺结核合并2型糖尿病的保护基因；可以推测DRB1＊09和DQB1＊05基因在肺结核合并2型糖尿病的发病中起一定作用，或是真正起作用的基因与它们连锁，有待于进一步研究。

Analysis of the association between HLA-DRB1

OBJECTIVE: To analyze the association between HLA DRB(1), DQB(1) allele and pulmonary tuberculosis complicated with type 2 diabetes mellitus among Han nationality of northern Chinese. METHODS: By using PCR-SSP technique, the genomic DNA typing was applied to compare the difference of the gene frequency between patients and normal controls. The relative risks (RR) of the disease were also estimated. 214 cases were observed, including 123 pulmonary tuberculosis complicated with type 2 diabetes mellitus, 45 type 2 diabetes mellitus, and 46 normal subjects. All these cases were respectively hospitalized in Beijing Thoracic Tumor & Tuberculosis Hospital, Tianjin Lung Disease Hospital, Beijing Thoracic Disease Hospital, Shijiazhuang Diabetes Hospital, from 1998 to 1999. RESULTS: The frequency of DRB(1) * 09 allele in pulmonary tuberculosis complicated with type 2 diabetes mellitus cases was significantly higher than that of DRB(1) * 09 allele in normal controls, 25.10% Vs 14.03%, RR = 2.22, the frequency of DRB(1) * 09 allele in cases was also significantly higher than that of DRB(1) * 09 allele in type 2 diabetes mellitus controls, 25.1% Vs 9.32%, RR = 3.16; the frequency of DQB(1) * 05 allele was significantly lower than that of DQB(1) * 05 allele in normal controls and diabetes mellitus, 7.17% Vs 19.24%, RR = 0.30, 7.17% Vs 21.12%, RR = 0.26. CONCLUSIONS: The results indicate that the DRB(1) * 09 allele is susceptive to the pulmonary tuberculosis complicated with type 2 diabetes mellitus, the DQB(1) * 05 may be protective to the pulmonary tuberculosis complicated with diabetes mellitus. The DRB(1) * 09 allele and DQB(1) * 05 allele may affect the incidence of the pulmonary tuberculosis complicated with type 2 diabetes mellitus, or real effect genes link with them.