| 2种方法检测食品中沙门菌的实验研究 |
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| 引用本文： | 刘玉兰. 2种方法检测食品中沙门菌的实验研究[J]. 预防医学论坛, 2010, 0(4) |
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| 作者姓名： | 刘玉兰 |
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| 作者单位： | 府谷县疾病预防控制中心; |
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| 摘 要： | [目的]比较自动酶联荧光免疫分析系统(VIDAS)和常规细菌培养法(GB)检测食品中沙门菌的效果。[方法]2009年7月,使用VIDAS法和常规细菌培养法平行检测各种样品中沙门菌,以常规细菌培养法作参照,对VIDAS技术进行评价。[结果]160份样品中,VIDAS法检出15份阳性样品,阳性率为9.38%,分离出12株沙门菌株;常规细菌培养法检出12份阳性样品,阳性率为7.50%,分离出12株沙门菌株。[结论]VIDAS法敏感、特异、简便、快速,可作为食品中沙门菌的快速筛检,常规培养法主要作为沙门菌的确证试验。
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| 关 键 词： | 沙门氏菌 VIDAS 快速检测 常规培养法 |
Comparative Study of the Examination of Salmonella in Food with two Methods |
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| LIU Yu-lan.. Comparative Study of the Examination of Salmonella in Food with two Methods[J]. Preventive Medicine Tribune, 2010, 0(4) |
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| Authors: | LIU Yu-lan. |
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| Affiliation: | LIU Yu-lan.(Fugu County Center for Disease Control , Prevention,Fugu,719400,Shanxi,China) |
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| Abstract: | [Objective]To compare the examination effect of Salmonella in food with VIDAS automatic enzyme-linked fluorescent immunoassay and routine bacterial culture (GB).[Methods]Salmonella in food samples was tested with VIDAS and GB in July 2009,VIDAS technique was evaluated based on the method of routine bacterial culture.[Results]Of the 160 samples tested,15 of them were positive detected with VIDAS method and the positive rate was 9.38%,12 Salmonella strains were isolated.While 12 of them were positive detected... |
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| Keywords: | Salmonella VIDAS Rapid detection Routine culture method |
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